Corn steep liquor (CSL) is a major by-product of the corn wet milling industry, widely employed as a cost-effective nutrient source in industrial fermentations.However, the underlying mechanisms by which CSL affected the fermentation performance remain largely unclear.This study observed that the addition of CSL at varying concentrations facilitated the fermentative production of L-homoserine in Corynebacterium glutamicum.To explore the effects of adding CSL on genes and metabolic pathways related to the L-homoserine synthesis pathway, transcriptome analysis was used to explore its potential novel genes and finally verified by quantitative reverse transcription polymerase chain reaction.Results revealed that the cg3096, coding for an aldehyde dehydrogenase Ald, was essential for effectively promoting the fermentation of CSL to produce L-homoserine.This efficient production of L-homoserine may be partially attributed to the increased levels of intracellular NADPH supply.Further investigation implied that overexpression of cg3096 also resulted in increased expression levels of some substrate transport (amt, cg0922, urtABC) and utilization genes (cg0535-cg0536).Collectively, the findings revealed the essential function and possible mechanism of cg3096 in achieving a high level of L-homoserine production, providing new perspectives on the effectiveness of CSL in fermentation.
QI Yuting
,
ZHONG Zhaoyue
,
ZHANG Xia
,
XU Ning
,
WEI Liang
,
LIU Jun
,
JIANG Juquan
,
SHAO Li
. Investigation of aldehyde dehydrogenase Ald by transcriptome analysis toward enhancing L-homoserine production in Corynebacterium glutamicum[J]. Food and Fermentation Industries, 2025
, 51(6)
: 8
-14
.
DOI: 10.13995/j.cnki.11-1802/ts.039156
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