Glycogen-like- polymer is synthesized by biological enzyme method in vitro and is widely used in biology, medical treatment, chemical industry and other fields.Its small molecular size (the molecular weight is 106 Da and below) makes it have good application effect.In this study, glycogen-like- polymer with molecular weight of 106 Da was synthesized by the synergistic method of sucrose phosphorylase-α-glucan phosphorylase-branching enzyme-4-α-Glycosyltransferase.A series of glycogen-like- polymer samples with different molecular sizes and fine structures were synthesized by controlling the ratio of enzyme activities of reaction elements.The high-performance gel size exclusion chromatography, high-performance anion chromatography and nuclear magnetic resonance hydrogen spectrum were used respectively to characterize the molecular size and fine structure.The results showed that the molecular weight of Glycogen-like- polymer reduce from 2.20×107 Da to 4.94×106 Da successfully and finally stabilize at 106 Da.The average chain length is between 8.11 and 9.99.The branching degree remained at 7.4%-10.7% showed it retain the highly branched structure.The particle size Rz was between 19.3 nm and 28.0 nm and the anti-enzymatic hydrolysis rate was between 39.78% and 43.19%.This provides a new idea for the in vitro enzymatic synthesis of glycogen-like-polymer, and also increases the application potential in biomedical fields.
DONG Hui
,
DONG Jingjing
,
JIN Zhengyu
,
LI Xiaoxiao
,
BAI Yuxiang
. Enzymatic synthesis of glycogen-like-polymer and the fine structure analysis of the product[J]. Food and Fermentation Industries, 2025
, 51(7)
: 1
-8
.
DOI: 10.13995/j.cnki.11-1802/ts.039379
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