This study investigated six human-derived Bifidobacterium species using a synthetic medium with intestinal mucin as the sole carbon source, to analyze their mucin utilization characteristics and interspecies cross-feeding relationships.Results indicated that only Bifidobacterium bifidum could utilize mucin in vitro and foster the growth of other bifidobacteria.Building on this, non-targeted metabolomics was employed to examine the mucin utilization byproducts of B. bifidum, with subsequent KEGG pathway enrichment of the identified differential metabolites.The findings revealed strain-specific differential metabolites (Variable importance in the projection>1, P<0.05, Fold change>1.5) post mucin utilization by B. bifidum, with strain FFJND15M5 exhibiting the most significant concentration increase in 22 metabolites.B. bifidum was capable of producing derivative sugars like N-acetylneuraminic acid, L-fucose, and N-acetyl-D-galactosamine 4-sulfate, as well as metabolites such as succinic acid, 3-phenyllactic acid, DL-4-hydroxyphenyllactic acid, and indole-3-lactic through various metabolic pathways including pyrimidine metabolism, amino sugar and nucleotide sugar metabolism, and butanoate metabolism.These results provide a scientific theoretical foundation for understanding the mucin utilization behavior of B. bifidum.
XIN Zongyuan
,
LI Wentian
,
LIN Guopeng
,
GUO Min
,
TIAN Peijun
,
WANG Linlin
,
JIN Xing
,
WANG Gang
,
ZHANG Hao
. Detection of Bifidobacterium’s ability to utilize intestinal mucin and metabolomic analysis of its metabolites[J]. Food and Fermentation Industries, 2025
, 51(7)
: 16
-24
.
DOI: 10.13995/j.cnki.11-1802/ts.039090
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