Food and Fermentation Industries >

2025 , Vol. 51 >Issue 7: 32 - 39

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037411

Antibacterial mechanism against Listeria monocytogenes by antimicrobial peptide PNMGL2 from Lactiplantibacillus plantarum

  • REN Qingxia ,
  • ZHOU Zengjia ,
  • WU Jingwei ,
  • YANG Zhennai
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  • (Key Laboratory of Geriatric Nutrition and Health of Ministry of Education, Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China)

Received date: 2023-09-18

  Revised date: 2024-05-16

  Online published: 2025-04-29

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Abstract

Listeria monocytogenes is a food-borne pathogenic bacterium, which can cause serious food public health security problems.The antimicrobial peptide PNMGL2 from Lactiplantibacillus plantarum has obvious inhibition on the foodborne pathogenic bacteria, but its antibacterial mechanism is unclear.Time-kill assays showed that PNMGL2 could effectively inhibit the growth of L.monocytogenes.The propidium iodide (PI) uptake and release of lactate dehydrogenase (LDH) experiments suggested that PNMGL2 could damage the integrity of cell membrane of L.monocytogenes.Furthermore, observation by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser-scanning microscopy (CLSM) indicated that PNMGL2 induced visual cell damage, promoted the formation of pores, destroyed the integrity of cell membrane, increased the permeability of membrane, and triggered leakage of intracellular substances (phosphorus, potassium, ATP, proteins, and nucleic acids).In addition, PNMGL2 could inhibit biofilm formation of L.monocytogenes and hemolytic activity of exotoxins.This study preliminarily elucidated the antibacterial mechanism of PNMGL2 against L.monocytogenes, providing a theoretical basis for the development and utilization of PNMGL2 as a new food antimicrobial agent.

Key words： antimicrobial peptide; antibacterial mechanism; Listeria monocytogenes; membrane integrity

Cite this article

REN Qingxia , ZHOU Zengjia , WU Jingwei , YANG Zhennai . Antibacterial mechanism against Listeria monocytogenes by antimicrobial peptide PNMGL2 from Lactiplantibacillus plantarum[J]. Food and Fermentation Industries, 2025 , 51(7) : 32 -39 . DOI: 10.13995/j.cnki.11-1802/ts.037411

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