Steviol glycosides are a natural, non-nutritive, high-intensity sweetener with functional properties, known as the world’s third sugar source.Stevioside is the main sweetening component of Steviol glycosides, with its molecular structure containing three glycosidic bonds available for hydrolysis.The specific hydrolysis of the glycosidic bond in stevioside can yield functional glycosides with varying sweetness and properties.This article first discovered that lipase A from Aspergillus niger can specifically hydrolyze stevioside to prepare rubusoside.This study investigated the effects of factors such as temperature, pH, substrate concentration, enzyme dosage, metal ions, etc., on the enzymatic reaction with a single-factor optimization method, and conducted a preliminary analysis of the reaction pathway.Results indicated that lipase A exhibited high specificity towards stevioside, with the main hydrolysis product being rubusoside.Under the optimal conditions of 55 ℃, pH 4.5, and an enzyme dosage of 1 200 U/g, the conversion rate of stevioside could reach 99% after a 9-hour reaction, with a rubusoside yield of 90%.This study expanded the preparation methods of rubusoside, which is of significant importance for the in-depth application of stevia glycosides.
XIE Yingchun
,
LIU Yutong
,
CHEN Junming
,
WANG Cheli
. Study on hydrolytic activity of lipase A specific catalytic stevioside[J]. Food and Fermentation Industries, 2025
, 51(7)
: 58
-63
.
DOI: 10.13995/j.cnki.11-1802/ts.039086
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