In this study, Limosilactobacillus fermentun WHH2483 isolated from 168 lactic acid bacterial strains originating from traditional fermented foods, showed potential for caries prevention.This strain demonstrated the ability to inhibit the growth and biofilm formation of Streptococcus mutans ATCC25175, with an inhibition zone diameter of (21.20±1.42) mm and a biofilm inhibition rate of (95.05±3.25)%.WHH2483 also showed good oral colonization potential, with an auto-aggregation rate of (39.19±1.65)% and a co-aggregation rate of (77.08±1.08)%, while exhibiting low adhesion to hydroxyapatite [adhesion rate of (20.72±1.20)%].A dental caries model in SD rats was established to validate the caries-preventive function of the strain.WHH2483 can significantly inhibit the proliferation of Streptococcus mutans in the oral cavity of rats, increase the loss of enamel surface and volume, reduce the levels of caries, maintain the integrity of tooth structure, and reduce the occurrence and development of caries.This study provides foundational support for the development and application of L. fermentum WHH2483 as a probiotic for preventing dental caries.
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