To investigate the hydrogen production performance of Clostridium spp. as an efficient hydrogen-producing fermentation strain, a strain named WZ-1, identified as Clostridium guangxiense, was isolated from the pit mud of Nongxiangxing Baijiu due to its hydrogen-producing ability.Under anaerobic conditions at 35 ℃, strain WZ-1 demonstrated the capacity to produce high levels of hydrogen (58.99%) and butyric acid (1 960 mg/L) through fermentation on a medium with glucose as the substrate.To comprehensively analyze its functional properties, the genome of strain WZ-1 was sequenced and annotated.Genomic analysis revealed that the total length of the WZ-1 genome was 4 406 933 bp with a guanine cytosine content of 31.95%, including 4 172 coding sequences, 3 rRNA genes, and 52 tRNA genes.Analysis of KEGG metabolic pathways of the strain revealed hydrogen production during pyruvate decarboxylation, hydrogen production by NADH+H+ redox balance regulation, hydrogen production by carbon monoxide, hydrogen production by nitrogen fixation, and its butyric acid-producing pathway in WZ-1.The results of this study analyzed the hydrogen and butyric acid production characteristics of WZ-1 at the genomic level and indicated that WZ-1 holds promising application prospects in the fields of hydrogen production, butyric acid production, and promoting circular economy initiatives.Furthermore, these findings provide valuable insights for the development of clean energy production technologies.
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