Food and Fermentation Industries >

2025 , Vol. 51 >Issue 7: 159 - 166

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038548

Antibacterial activity of pomegranate peel extract against Bacillus cereus and its effect on cell membrane

  • YU Fang ,
  • WANG Qianning ,
  • TIAN Huanhuan ,
  • LYU Xiong ,
  • ZHANG Junxia
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  • 1(College of Forestry, Gansu Agricultural University, Lanzhou 730070, China)
    2(Faculty of Bioengineering and Technology, Tianshui Normal University, Tianshui 741001, China)

Received date: 2024-01-10

  Revised date: 2024-04-15

  Online published: 2025-04-29

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Abstract

Pomegranate peel was used as a test material to study its antibacterial activity against the food-borne putrefactive bacterium Bacillus cereus and its effect on cell membrane permeability and integrity.This study provides a theoretical basis for the development of safe plant-derived preservatives and deep utilisation of pomegranate peel.The antibacterial activity showed that the ethanol extract of pomegranate peel inhibited Bacillus cereus better than Bacillus subtilis and Bacillus atrophicus, and the minimum inhibitory concentrationof pomegranate peel extract against Bacillus cereus was 1 mg/mL.The pomegranate peel extract had a significant antibacterial effect on the overall biomass of the three kinds of Bacillus, and the total soluble sugars, soluble proteins, nucleic acid leakage, and electrical conductivity of the treatment group were significantly higher than that of the control, and their effects were enhanced with the increase of pomegranate peel extract concentration.The treatment of high concentration of pomegranate peel extract increased the total soluble sugar content of Bacillus cereus to 1.75 times of the control, the soluble protein content to 2.43 times, the nucleic acid leakage reached the peak at 6 h, which was 3.04 times higher compared to the control, and the electrical conductivity was 1.16 times higher, and the differences between the high concentration treatment groups and the control were all highly significant (P＜0.01).Combined with the scanning electron microscopy, the bacterial cells appeared to collapse and crumple, the cell boundary was blurred, the bacterial cells became shorter and thinner, and the cell contents overflowed, which proved that the pomegranate peel extract changed the morphology of the bacterial cells and destroyed the cell membranes, causing the leakage of the soluble total sugars, soluble proteins, nucleic acids, and other contents, achieving the antibacterial effect.

Key words： pomegranate peel; Bacillus cereus; bacteriostatic effects; food microorganisms; cell membrane

Cite this article

YU Fang , WANG Qianning , TIAN Huanhuan , LYU Xiong , ZHANG Junxia . Antibacterial activity of pomegranate peel extract against Bacillus cereus and its effect on cell membrane[J]. Food and Fermentation Industries, 2025 , 51(7) : 159 -166 . DOI: 10.13995/j.cnki.11-1802/ts.038548

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