Genome analysis and enzymology study of Bacillus subtilis HB-011

  • JI Shuai ,
  • WANG Xinyu ,
  • SUN Jiajia ,
  • ZHANG Xiaotong ,
  • ZHANG Senglong ,
  • ZHANG Zhi
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  • 1(College of Life Sciences, Northeast Forestry University, Harbin 150040,China)
    2(Shanghai Municipal Engineering Design and Research Institute (Group) Co. Ltd., Shanghai 200092,China)
    3(Heilongjiang Guohong Energy Saving and Environmental Protection Co. Ltd., Qiqihar 161000, China)

Received date: 2024-02-05

  Revised date: 2024-04-30

  Online published: 2025-05-28

Abstract

This study screened a strain of laccase-producing bacteria with high-efficiency lignin degradation ability from the natural environment by using enzyme activity as an evaluation index and the phenol blue decolorization method.Physiological and biochemical indicators and 16S rDNA molecular biology identification results showed that this strain was Bacillus subtilis, named Bacillus subtilis HB-011.Through the annotation of the Bacillus subtilis HB-011 whole gene analysis in the GO, COG, KEGG, and CAZY databases, the related pathways for its degradation of lignin were preliminarily speculated.The enzymology property research experiment showed that the fermented laccase had good stability under the conditions of pH 2-6 and temperature 20-55 ℃.The strain Bacillus subtilis HB-011 had good application potential in the degradation of lignin.This study provides strain reserves and theoretical basis for the research of high-efficiency degradation of lignin laccase.

Cite this article

JI Shuai , WANG Xinyu , SUN Jiajia , ZHANG Xiaotong , ZHANG Senglong , ZHANG Zhi . Genome analysis and enzymology study of Bacillus subtilis HB-011[J]. Food and Fermentation Industries, 2025 , 51(9) : 40 -49 . DOI: 10.13995/j.cnki.11-1802/ts.038842

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