Food and Fermentation Industries >

2025 , Vol. 51 >Issue 9: 50 - 58

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039507

Comparative genomic analysis of Lactococcus garvieae in pit mud of Baijiu

  • ZHAO Zhuoru ,
  • CHEN Xiaosong ,
  • CHEN Cong ,
  • ZOU Wei
Expand
  • 1(College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644005, China)
    2(Lab of Baijiu Resource Microorganisms & Big Data, Sichuan University of Science & Engineering, Yibin 644005, China)

Received date: 2024-04-09

  Revised date: 2024-05-08

  Online published: 2025-05-28

Fold

Abstract

The Lactococcus garvieae is a functionally significant microorganism widely present in the Baijiu brewing ecosystem.It not only produces diverse flavor compounds during fermentation but also plays a pivotal role in maintaining the ecological balance of the brewing system. The study annotated the functional genes of four strains of L.garvieae isolated from pit mud environments, conducted a pan-genome analysis in combination with other ecological niches of L.garvieae to explore their population characteristics in phylogenetic development and individual specificities in the Baijiu environment.The results revealed an open state of the L.garvieae pan-genome, with the core genome primarily involved in basic metabolic functions of the bacteria.The KEGG annotation of the core genome demonstrates that the L.garvieae genome possesses the ability to produce cellulases, glycosidases, and esterases, enzymes that facilitate microbial growth.Additionally, it generated precursor substances, including lactic acid, acetic acid, and ethanol.These precursors were catalyzed by esterase activity, resulting in the formation of lactic ethyl ester and acetic ethyl ester.Virulence factor analysis indicated that the four strains of L.garvieae isolated from pit mud environment possess safety at the genomic level, but may be potentially influenced by external regulatory factors.Substantial horizontal gene transfer was observed in L.garvieae.The findings of this study reveal the genetic diversity and evolutionary characteristics of L.garvieae, providing a theoretical basis for its application in the Baijiu industry.

Key words： Lactococcus garvieae; pan-genome analysis; lactic acid bacteria; core genome; Baijiu

Cite this article

ZHAO Zhuoru , CHEN Xiaosong , CHEN Cong , ZOU Wei . Comparative genomic analysis of Lactococcus garvieae in pit mud of Baijiu[J]. Food and Fermentation Industries, 2025 , 51(9) : 50 -58 . DOI: 10.13995/j.cnki.11-1802/ts.039507

References

[1] LIN Y S, KWEH K H, KOH T H, et al.Genomic analysis of Lactococcus garvieae isolates[J].Pathology, 2020, 52(6):700-707.
[2] 阎春悦, 利佳炜, 张茜, 等.不同香型白酒酿造过程乳酸菌的研究进展[J].酿酒科技, 2023(9):114-120.
YAN C Y, LI J W, ZHANG Q, et al.Research progress in lactic acid bacteria in the production of Baijiu of different flavor types[J].Liquor-Making Science & Technology, 2023(9):114-120.
[3] 吴正坤, 缪礼鸿, 张明春, 等.不同贮存期高温大曲中乳酸菌的多样性及其耐受性分析[J].中国酿造, 2018, 37(6):61-67.
WU Z K, MIAO L H, ZHANG M C, et al.Diversity and tolerance of lactic acid bacteria in high temperature Daqu at different storage periods[J].China Brewing, 2018, 37(6):61-67.
[4] 刘凡, 仇钰莹, 周新虎, 等.洋河浓香型白酒酒醅中产酸细菌与有机酸合成的相关性研究[J].食品与发酵工业, 2018, 44(12):22-29.
LIU F, QIU Y Y, ZHOU X H, et al.The correlation between organic acid producing bacteria and organic acids biosynthesis in fermented grains of Yanghe strong-aroma spirit[J].Food and Fermentation Industries, 2018, 44(12):22-29.
[5] 胡世伟, 王超凡, 王景春, 等.浓香型白酒窖泥微生物群落及其风味成分研究进展[J].中国酿造, 2023, 42(12):9-12.
HU S W, WANG C F, WANG J C, et al.Research progress of microbial community and flavor substances in strong-flavor Baijiu pit mud[J].China Brewing, 2023, 42(12):9-12.
[6] 陈聪, 邹伟, 汤秀娟, 等.产酯酶格氏乳球菌的筛选、鉴定与基因组注释[J].食品科学, 2024, 45(8):87-95.
CHEN C, ZOU W, TANG X J, et al.Screening, identification and genome annotation of esterase-producing Lactococcus garvieae[J].Food Science, 2024, 45(8):87-95.
[7] 骆红波, 孙优兰, 范奇高, 等.组学技术在白酒酿造微生物中的应用进展[J].中国酿造, 2023, 42(12):13-21.
LUO H B, SUN Y L, FAN Q G, et al.Progress on the application of omics technologies in Baijiu brewing microorganism[J].China Brewing, 2023, 42(12):13-21.
[8] ZHOU X G, REN L F, LI Y T, et al.The next-generation sequencing technology:A technology review and future perspective[J].Science China.Life Sciences, 2010, 53(1):44-57.
[9] 余晶晶, 黄永光.基因组学及其在白酒酿造基础研究领域中的应用[J].食品与发酵工业, 2021, 47(7):278-284.
YU J J, HUANG Y G.Genomics and its application in basic research field of Baijiu brewing[J].Food and Fermentation Industries, 2021, 47(7):278-284.
[10] TETTELIN H, MASIGNANI V, CIESLEWICZ M J, et al.Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae:Implications for the microbial “pan-genome”[J].Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(39):13950-13955.
[11] 何苗, 赵雨晴, 李居行, 等.弯曲乳杆菌与清酒乳杆菌的比较基因组学分析[J].食品科学, 2024, 45(2):48-56.
HE M, ZHAO Y Q, LI J X, et al.Comparative genomic analysis of Latilactobacillus curvatus and L.sakei[J].Food Science, 2024, 45(2):48-56.
[12] TATUSOVA T, DICUCCIO M, BADRETDIN A, et al.NCBI prokaryotic genome annotation pipeline[J].Nucleic Acids Research, 2016, 44(14):6614-6624.
[13] AZIZ R K, BARTELS D, BEST A A, et al.The RAST Server:Rapid annotations using subsystems technology[J].BMC Genomics, 2008, 9:75.
[14] CHAUDHARI N M, GUPTA V K, DUTTA C.BPGA- an ultra-fast pan-genome analysis pipeline[J].Scientific Reports, 2016, 6:24373.
[15] ZHAO Y B, JIA X M, YANG J H, et al.PanGP:A tool for quickly analyzing bacterial pan-genome profile[J].Bioinformatics, 2014, 30(9):1297-1299.
[16] RICHTER M, ROSSELLÓ-MÓRA R, OLIVER GLÖCKNER F, et al.JSpeciesWS:A web server for prokaryotic species circumscription based on pairwise genome comparison[J].Bioinformatics, 2016, 32(6):929-931.
[17] KUMAR S, STECHER G, LI M, et al.MEGA X:Molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution, 2018, 35(6):1547-1549.
[18] KANEHISA M, SATO Y, KAWASHIMA M, et al.KEGG as a reference resource for gene and protein annotation[J].Nucleic Acids Research, 2016, 44(D1):D457-D462.
[19] HUERTA-CEPAS J, SZKLARCZYK D, HELLER D, et al.eggNOG 5.0:A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses[J].Nucleic Acids Research, 2019, 47(D1):D309-D314.
[20] CHEN L H, YANG J, YU J, et al.VFDB:A reference database for bacterial virulence factors[J].Nucleic Acids Research, 2005, 33(Database issue):D325-D328.
[21] BERTELLI C, LAIRD M R, WILLIAMS K P, et al.IslandViewer 4:Expanded prediction of genomic islands for larger-scale datasets[J].Nucleic Acids Research, 2017, 45(W1):W30-W35.
[22] ARNDT D, GRANT J R, MARCU A, et al.PHASTER:A better, faster version of the PHAST phage search tool[J].Nucleic Acids Research, 2016, 44(W1):W16-W21.
[23] PARKS D H, IMELFORT M, SKENNERTON C T, et al.CheckM:Assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes[J].Genome Research, 2015, 25(7):1043-1055.
[24] ALIKHAN N F, PETTY N K, BEN ZAKOUR N L, et al.BLAST ring image generator (BRIG):Simple prokaryote genome comparisons[J].BMC Genomics, 2011, 12:402.
[25] MORIYA Y, ITOH M, OKUDA S, et al.KAAS:An automatic genome annotation and pathway reconstruction server[J].Nucleic Acids Research, 2007, 35(Web Server issue):W182-W185.
[26] BOBER M, MÖRGELIN M, OLIN A I, et al.The membrane bound LRR lipoprotein Slr, and the cell wall-anchored M1 protein from Streptococcus pyogenes both interact with type I collagen[J].PLoS One, 2011, 6(5):e20345.
[27] XU S Y, LIU Y, GAO J, et al.Comparative genomic analysis of Streptococcus dysgalactiae subspecies dysgalactiae isolated from bovine mastitis in China[J].Frontiers in Microbiology, 2021, 12:751863.
[28] 贾伟娟, 宋丽丽, 张玲艳, 等.蜡样芽胞杆菌毒素的最新研究进展[J].中国抗生素杂志, 2022, 47(6):534-542.
JIA W J, SONG L L, ZHANG L Y, et al.Research progress of the toxins from Bacillus cereus[J].Chinese Journal of Antibiotics, 2022, 47(6):534-542.
[29] HAAS W, SHEPARD B D, GILMORE M S.Two-component regulator of Enterococcus faecalis cytolysin responds to quorum-sensing autoinduction[J].Nature, 2002, 415(6867):84-87.
[30] SEBAIHIA M, PECK M W, MINTON N P, et al.Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes[J].Genome Research, 2007, 17(7):1082-1092.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.