Influence of Kefir yeast on growth and metabolism of Lactobacillus kefiri

  • LI Shiwei ,
  • BAI Ying
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  • (School of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China)

Received date: 2024-03-22

  Revised date: 2024-05-23

  Online published: 2025-05-28

Abstract

This study investigated the interactions between a high-exopolysaccharide-producing Lactobacillus kefiri (L63) isolated from kefir and two different yeasts derived from kefir.Using qPCR to amplify characteristic sequences of L63, the growth status during monoculture and coculture was determined.Results revealed that Kluyveromyces marxianus Y4 initially promoted the growth of L63, while both yeasts inhibited its growth in the later stages of fermentation.The phenol-sulfuric acid method was employed to measure differences in exopolysaccharide (EPS) production during monoculture and coculture.It was observed that coculturing with either yeast significantly increased EPS production (P<0.01).During the co-fermentation of skim milk, it was found that coculturing with both yeasts significantly increased acidity in the early fermentation stage, while in the later stage, results tended to align with a monoculture of L63.Metabolomic analysis revealed differential metabolite distribution during coculture, primarily associated with cell growth, energy metabolism, and respiratory processes, closely linked to the growth status of the cells, thereby partially elucidating the reasons for the observed changes in growth status during coculture of L63.

Cite this article

LI Shiwei , BAI Ying . Influence of Kefir yeast on growth and metabolism of Lactobacillus kefiri[J]. Food and Fermentation Industries, 2025 , 51(9) : 67 -75 . DOI: 10.13995/j.cnki.11-1802/ts.039320

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