Achyranthes bidentata polysaccharide (AP) is a functional compound in A. bidentata with diverse pharmacological effects, including antitumor and immunomodulatory properties.However, the impact of AP on the intestinal health of lung cancer patients remains unclear.To investigate the gastrointestinal digestive properties of AP and its influence on the gut microbiota in lung cancer patients by utilizing an in vitro simulation of the gastrointestinal digestive system and the fecal fermentation model tailored to lung cancer patients.These findings showed that the molecular weight of AP remained relatively unchanged following simulated digestion by oral, gastric, and small intestinal fluids in vitro, reflecting it could transit to the colon in a more intact form.After fermented by fecal flora of patients with lung cancer, it regulated the structures and compositions of gut microbiota in the fermentation fluid, promoting the growth of beneficial bacterial genera such as Prevotella, Phascolarctobacterium, Akkermansia, and others while inhibiting the proliferation of Ruminococcus and Sutterella.Meanwhile, AP promoted the production of acetic acid, propionic acid, and butyric acid.In addition, functional predictions indicated that AP improved microbial metabolic function, promoted amino acid biosynthesis, pantothenic acid and coenzyme A metabolism, and had the potential to inhibit the development of lung cancer.In conclusion, the ability of AP to modulate the gut microbiota and promote the production of short-chain fatty acids in lung cancer patients may provide new ideas for the prevention and mitigation of lung cancer.
MA Wen
,
SHEN Fanglin
,
LIU Chang
,
ZHU Hongkang
,
WANG Cheng
,
SHEN Jian
,
ZHAO Yong
,
QIAN He
. Impact of Achyranthes bidentata polysaccharide on the gut microbiota of lung cancer patients based on in vitro digestion and fermentation models[J]. Food and Fermentation Industries, 2025
, 51(9)
: 114
-123
.
DOI: 10.13995/j.cnki.11-1802/ts.039635
[1] THAI A A, SOLOMON B J, SEQUIST L V, et al.Lung cancer[J].The Lancet, 2021, 398(10299):535-554.
[2] CORRÊA-OLIVEIRA R, FACHI J L, VIEIRA A, et al.Regulation of immune cell function by short-chain fatty acids[J].Clinical & Translational Immunology, 2016, 5(4):e73.
[3] ZHENG Y J, FANG Z Y, XUE Y, et al.Specific gut microbiome signature predicts the early-stage lung cancer[J].Gut Microbes, 2020, 11(4):1030-1042.
[4] CHAI J H, HE T T, JIANG S L, et al.Oligo/polysaccharides from Cyathula officinalis and Achyranthes bidentata:A review of structures and bioactivities[J].Journal of Pharmacy and Pharmacology, 2024, 76(4):307-326.
[5] GUO Y X, CHEN X F, GONG P, et al.Advances in the in vitro digestion and fermentation of polysaccharides[J].International Journal of Food Science & Technology, 2021, 56(10):4970-4982.
[6] FAN S R, WANG Y X, ZHANG Y, et al.Achyranthes bidentata polysaccharide activates nuclear factor-kappa B and promotes cytokine production in J774A.1 cells through TLR4/MyD88 signaling pathway[J].Frontiers in Pharmacology, 2021, 12:753599.
[7] BRODKORB A, EGGER L, ALMINGER M, et al.INFOGEST static in vitro simulation of gastrointestinal food digestion[J].Nature Protocols, 2019, 14(4):991-1014.
[8] LIU C, GUO Y H, CHENG Y L, et al.A colon-targeted delivery system of torularhodin encapsulated in electrospinning microspheres, and its co-metabolic regulation mechanism of gut microbiota[J].Food Hydrocolloids, 2023, 135:108189.
[9] PÉREZ-BURILLO S, MOLINO S, NAVAJAS-PORRAS B, et al.An in vitro batch fermentation protocol for studying the contribution of food to gut microbiota composition and functionality[J].Nature Protocols, 2021, 16(7):3186-3209.
[10] LIU C, DU P, CHENG Y L, et al.Study on fecal fermentation characteristics of aloe polysaccharides in vitro and their predictive modeling[J].Carbohydrate Polymers, 2021, 256:117571.
[11] LIU C, JIANG W H, YANG F W, et al.The combination of microbiome and metabolome to analyze the cross-cooperation mechanism of Echinacea purpurea polysaccharide with the gut microbiota in vitro and in vivo[J].Food & Function, 2022, 13(19):10069-10082.
[12] TETT A, PASOLLI E, MASETTI G, et al.Prevotella diversity, niches and interactions with the human host[J].Nature Reviews.Microbiology, 2021, 19(9):585-599.
[13] LI Q Q, SUN X, YU K Y, et al.Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance[J].Cell Reports, 2022, 40(9):111308.
[14] DEROSA L, ROUTY B, THOMAS A M, et al.Intestinal Akkermansia muciniphila predicts clinical response to PD-1 blockade in patients with advanced non-small-cell lung cancer [J].Nature Medicine, 20, 28(2):315-324.
[15] SHETTY S A, MARATHE N P, LANJEKAR V, et al.Comparative genome analysis of Megasphaera sp.reveals niche specialization and its potential role in the human gut[J].PLoS One, 2013, 8(11):e79353.
[16] ZHANG J C, GUO Z, XUE Z S, et al.A Phylo-functional core of gut microbiota in healthy young Chinese cohorts across lifestyles, geography and ethnicities[J].The ISME Journal, 2015, 9(9):1979-1990.
[17] HALL A B, YASSOUR M, SAUK J, et al.A novel Ruminococcus gnavus clade enriched in inflammatory bowel disease patients[J].Genome Medicine, 2017, 9(1):103.
[18] KAAKOUSH N O.Sutterella species, IgA-degrading bacteria in ulcerative colitis[J].Trends in Microbiology, 2020, 28(7):519-522.
[19] SCHWALM N D, GROISMAN E A.Navigating the gut buffet:Control of polysaccharide utilization in Bacteroides spp[J].Trends in Microbiology, 2017, 25(12):1005-1015.
[20] GROHMANN U, BRONTE V.Control of immune response by amino acid metabolism[J].Immunological Reviews, 2010, 236(1):243-264.
[21] ST PAUL M, SAIBIL S D, HAN S, et al.Coenzyme A fuels T cell anti-tumor immunity[J].Cell Metabolism, 2021, 33(12):2415-2427.
[22] BACH KNUDSEN K E, LÆRKE H N, HEDEMANN M S, et al.Impact of diet-modulated butyrate production on intestinal barrier function and inflammation[J].Nutrients, 2018, 10(10):1499.
[23] KIM K, KWON O, RYU T Y, et al.Propionate of a microbiota metabolite induces cell apoptosis and cell cycle arrest in lung cancer[J].Molecular Medicine Reports, 2019, 20(2):1569-1574.
[24] SAM Q H, LING H, YEW W S, et al.The divergent immunomodulatory effects of short chain fatty acids and medium chain fatty acids[J].International Journal of Molecular Sciences, 2021, 22(12):6453.
[25] HU J M, LIN S L, ZHENG B D, et al.Short-chain fatty acids in control of energy metabolism[J].Critical Reviews in Food Science and Nutrition, 2018, 58(8):1243-1249.
