Food and Fermentation Industries >

2025 , Vol. 51 >Issue 9: 230 - 238

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040074

Preparation and structural characterization of phosvtin phosphopeptide peptide-zinc chelation

  • ZHANG Mingran ,
  • XUE Tianrui ,
  • LYU Binfei ,
  • LI Can ,
  • ZHANG Xiaowei
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  • (College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)

Received date: 2024-06-03

  Revised date: 2024-07-23

  Online published: 2025-05-28

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Abstract

The phosvitin phosphopeptide (PPP) was obtained through enzymatic hydrolysis of phosvitin (PV) and subsequently chelated with zinc to produce the phosvitin phosphopeptide-zinc chelate (PPP-Zn), aiming to develop safe and highly bioavailable zinc supplements.PV was initially subjected to a two-step enzymatic hydrolysis using alkaline protease and trypsin, followed by a third step involving α-chymotrypsin.The conditions for enzymatic hydrolysis were optimized based on the degree of hydrolysis to prepare PPP effectively.The preparation process of PPP-Zn was further optimized using response surface methodology, with the zinc chelation rate as an index parameter.The structure of PPP-Zn was characterized using Zeta potential analysis, fluorescence spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy.Results showed that the optimal conditions for preparing PPP were pH 7.50 and enzymolysis at 50 ℃ for 4 h with an addition of 8% α-chymotrypsin, resulting in a hydrolysis degree of (26.62±0.39)%.For PPP-Zn preparation, the optimum conditions included a mass ratio of zinc to peptide at 1∶90(g∶mmol), pH 5.20, and temperature at 60 ℃ for 60 min, achieving a zinc chelation rate of (94.18±0.38)%.Upon zinc chelation, both Zeta potential and fluorescence intensity decreased while FTIR spectra indicated that carboxyl oxygen atoms, amino nitrogen atoms, and phosphate groups within PPP served as primary sites for binding with zinc ions.The high content of zinc in PPP-Zn indicated its promising potential as a novel type of effective zinc supplement.

Key words： phosvitin phosphopeptide; peptide-zinc chelate; process optimization; structural characterization

Cite this article

ZHANG Mingran , XUE Tianrui , LYU Binfei , LI Can , ZHANG Xiaowei . Preparation and structural characterization of phosvtin phosphopeptide peptide-zinc chelation[J]. Food and Fermentation Industries, 2025 , 51(9) : 230 -238 . DOI: 10.13995/j.cnki.11-1802/ts.040074

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