Food and Fermentation Industries >

2025 , Vol. 51 >Issue 9: 319 - 326

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039216

Antimicrobial resistance phenotype and gene distribution characterization of Vibrio alginolyticus in aquatic products in Hangzhou

  • DENG Peiwei ,
  • ZHENG Shuangzhi ,
  • YAN Han ,
  • PANG Yi ,
  • SHEN Lei ,
  • ZHU Junli ,
  • LU Haixia
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  • 1(School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China)
    2(Institute of Food Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China)

Received date: 2024-03-18

  Revised date: 2024-05-23

  Online published: 2025-05-28

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Abstract

This study aimed to investigate the antimicrobial resistance phenotype and resistance gene carrying of Vibrio alginolyticus in aquatic products in Hangzhou and to provide a reference for the prevention and control of V. alginolyticus pollution in aquatic products and improve the safety of aquatic products.The drug resistance of 14 strains of V. alginolyticus isolated from commercially available aquatic productswas detected by drug-sensitive paper diffusion method, and the multiple antimicrobial resistance index (MARI) was calculated.PCR amplification was used to detect 8 classes of 21 drug resistance genes and 1 class of integron gene, and the correlation between antimicrobial resistance phenotype and genotype of V. alginolyticus was analyzed.Results showed that fourteen strains of V. alginolyticus showed 14 antimicrobial resistance phenotypes, and all isolates were resistant to penicillin and amoxicillin, followed by cefazolin and ampicillin, which were resistant to 13 isolates respectively.A total of 13 isolates were sensitive to chloramphenicol and flufenicol, and all strains were sensitive to meropenem.The MARI of 11 isolates exceeded 0.2, accounting for 78.57% of the total.All isolates were detected to carry 3 or more antimicrobial resistance genes at the same time, sulfamide resistance gene sul1 and aminoglycoside resistance gene strB had the highest detection rates, respectively, detected in 13 isolates (92.86%), followed by tetracycline drug resistance gene tetA (85.71%), and the antimicrobial resistance genotypes were not completely consistent with the antimicrobial resistance phenotypes.This study showed that the detection rate of V. alginolyticus in aquatic products collected in Hangzhou was 13.3%.Most of the isolated strains had multiple antimicrobial resistance, and all isolates carried resistance genes of class 2 or above antibiotics, which was related to the pollution of the growth environment by antibiotics.Therefore, it is necessary to pay more attention to and standardize the rational use of antibiotics to prevent or reduce the occurrence of food safety incidents.

Key words： Vibrio alginolyticus; antimicrobial resistance gene; antimicrobial resistance; multidrug-resistant; aquatic products

Cite this article

DENG Peiwei , ZHENG Shuangzhi , YAN Han , PANG Yi , SHEN Lei , ZHU Junli , LU Haixia . Antimicrobial resistance phenotype and gene distribution characterization of Vibrio alginolyticus in aquatic products in Hangzhou[J]. Food and Fermentation Industries, 2025 , 51(9) : 319 -326 . DOI: 10.13995/j.cnki.11-1802/ts.039216

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