Geranylgeraniol (GGOH) is a straight-chain diterpenoid and a crucial precursor for the synthesis of various drugs and natural terpenoids.To achieve the efficient production of geranylgeraniol, this study first enhanced the gene expression of the mevalonate pathway and constructed a strain which can produce 168.0 mg/L of geranylgeraniol.Secondly, by introducing exogenous geranylgeranyl diphosphate synthase and downregulating the squalene synthesis competition pathway, the supply of precursors was increased.Combined with knocking out different regulatory factors, the yield of geranylgeraniol reached 678.2 mg/L.Subsequently, by overexpressing the phosphopentose pathway gene and increasing the supply of acetyl CoA, more cofactors and precursors were provided for the synthesis of geranylgeraniol.Increasing the copy number of fusion genes further improved the yield of geranylgeraniol to 1 116.5 mg/L.Finally, in a 5 L bioreactor, the production of geranylgeraniol by fed-batch fermentation reached 11.3 g/L, which is currently the highest reported level.This study provides a reference for the industrial production of geranylgeraniol and serves as a promising platform for the biosynthesis of natural terpenoids.
YOU Xifeng
,
PENG Zheng
,
ZHANG Juan
. Metabolic engineering of Saccharomyces cerevisiae for efficient synthesis of geranylgeraniol[J]. Food and Fermentation Industries, 2025
, 51(8)
: 1
-7
.
DOI: 10.13995/j.cnki.11-1802/ts.039465
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