To investigate the intervention effect of pectin on exacerbated colitis induced by Porphyromonas gingivalis (Pg), mice were randomly assigned into three groups, including model group, Pg treatment group, and pectin treatment group.From days 1 to 7, all groups of mice were administered 2% DSS to establish a DSS-induced colitis mouse model.From days 1 to 14, mice in the Pg group and pectin group were orally gavaged with 1×108 CFU Pg.Additionally, mice in the pectin group received continuous pectin treatment at a dose of 200 mg/kg for 14 days.The body weight, spleen index, colon length, splenic immune T-cell status, colonic histopathology, serum cytokine levels, and intestinal microbiota of each group of mice were examined.Results showed that Pg exacerbated colitis symptoms in mice, including body weight loss, colon shortening and exacerbated colonic tissue damage.Further experiments revealed increased splenic immune Th17 cells, elevated serum cytokine levels, and disrupted gut microbiota.Treatment with pectin alleviated colitis symptoms in mice, attenuating weight loss, colon shortening, and intestinal tissue damage, while also reducing serum cytokine levels and ameliorating disruption of gut microbiota.
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