This study aimed to investigate the effects of black tea on gastric tissue and metabolism in mice and to analyze the effects on stomach biorhythm in satiated and fasted mice.6-week-old C57BL/6 satiated and fasted mice were administered normal saline and black tea extract (450 mg/kg), respectively.30 min later, the gastric tissues and stomach contents of the mice were taken for RNA sequencing and metabolite detection, and the differences in transcriptomic and metabolomic levels of the gastric tissues of mice under different conditions of black tea intervention were detected.Results indicated that 907 genes were up-regulated and 810 genes were down-regulated after black tea intervention in the satiated group.The expression of 748 genes were up-regulated and 864 genes were down-regulated in the fasted group after black tea intervention.Enrichment analysis of KEGG pathways for the two groups indicated that there were significant differences in biological rhythm, lipid metabolism, glucose metabolism, and other related pathways.The detection of core genes related to biological rhythm showed that the expression of circadian clock genes Nr1d2, Per2, and Per3 were significantly up-regulated and Arntl expression was significantly down-regulated after black tea intervention in the satiated group and fasting group.Metabolism results of stomach contents showed that after black tea intervention, there were 1 355 up-regulated metabolites and 1 393 down-regulated metabolites in the stomach of mice in the satiated group.In the fasted group, there were 1 324 up-regulated metabolites and 1 378 down-regulated metabolites.The metabolites showed significant enrichment of the amino acid biosynthesis and purine pathways.These findings show that black tea may affect the biological rhythm of mice by regulating amino acid biosynthesis, stomach rhythm, and metabolism.Finally, it may also play a role in stomach digestion.
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