Food and Fermentation Industries >

2025 , Vol. 51 >Issue 10: 9 - 16

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039737

Expression of a heat-resistant transglutaminase in Streptomyces mobaraensis and its enzymatic property analysis

  • CHEN Zhiheng ,
  • ZHOU Can ,
  • WEI Zuoquan ,
  • TAN Yamei ,
  • YE Jiacai ,
  • CUI Shixiu ,
  • LIU Song
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  • 1(Science Center for Future Foods, Jiangnan University, Wuxi 214122, China)
    2(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    3(Jiaxing Institute of Future Food, Jiaxing 314000, China)

Received date: 2024-04-29

  Revised date: 2024-06-11

  Online published: 2025-06-11

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Abstract

Transglutaminase (TGase) which can catalyze protein crosslinking has been widely used in food processing.To promote its industrial application, Streptomyces mobaraensis which can efficiently produce heat-resistant TGase was successfully constructed in this study.First, this study synthesized the expression cassette of a TGase thermostable mutant TGm3 (1 745 bp), containing the natural promoter, pro-region, and terminator of S.mobaraensis TGase in addition to TGm3 coding sequence (optimized based on codon preference of streptomyces).Second, the expression cassette of TGm3 was integrated into the TGase gene locus of S.mobaraensis mutant smY2019-Δtg, yielding the TGm3-producing strain smY2019-TGm3.At last, the fermentation level of smY2019-TGm3 and the enzymatic properties of TGm3 were analyzed.As indicated by the shake flask fermentation, the extracellular TGase activity of smY2019-tgm3 reached the maximum value (19.5 U/mL) after 60 h fermentation.After being purified by the cation exchange column, the specific activity of TGm3 reached 41.76 U/mg, 2.7 times higher than that of wild-type TGase.The analysis of enzymatic properties showed that the 60 ℃ half-life (60.6 min) and the optimal temperature (60 ℃) of TGm3 were 33.6 times and 10 ℃ higher than those of wild-type TGase, respectively.These results show that smY2019-tgm3 can efficiently synthesize heat-resistant TGase and has a certain potential for industrial application.

Key words： Streptomyces mobaraensis; transglutaminase; efficient expression; thermostability

Cite this article

CHEN Zhiheng , ZHOU Can , WEI Zuoquan , TAN Yamei , YE Jiacai , CUI Shixiu , LIU Song . Expression of a heat-resistant transglutaminase in Streptomyces mobaraensis and its enzymatic property analysis[J]. Food and Fermentation Industries, 2025 , 51(10) : 9 -16 . DOI: 10.13995/j.cnki.11-1802/ts.039737

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