Constipation is a common gastrointestinal disease, and its incidence has obvious gender differences, in which women being more susceptible to constipation.Previous studies have found that the application of estrogen receptor β (ERβ) inhibitor (tamoxifen citrate) can successfully construct an animal model adapted to female constipation.Meanwhile, Bifidobacterium longum CCFM1240 has the effect of relieving female constipation, but the mechanism to exert its effect is still unknown.Based on that, this study intends to analyze the possible approaches of relieving female constipation from the perspective of repairing intestinal nerves and intestinal barriers.Results showed that compared with the model group, the intervention of B. longum CCFM1240 could significantly regulate the structure of intestinal microbiota (upregulate the relative abundance of Pediococcus, downregulate the relative abundance of Parabacterioids, Oscillibacter, Ruminococcus UGG-010, and Ruminiclostridium 9), increasing the content of six short chain fatty acids (especially acetic acid, butyric acid, and valeric acid), helping to restore the intestinal barriers and intestinal nervous system (promote the secretion of ACh by intestinal neurons, reduce the pro-inflammatory factor IL-6 and increase the proportion of Treg), and improving intestinal inflammatory response.This study demonstrates that B. longum CCFM1240 can balance the intestinal environment, repairing damaged intestinal barriers and intestinal nerves, thereby alleviate constipation in female rats induced by estrogen receptor beta inhibitors, and provide a new method to treat female constipation.
LI Jiazhen
,
MEI Chunxia
,
HUANG Yi
,
LIU Wenxu
,
ZHU Shengnan
,
XUE Yifan
,
WANG Gang
,
WANG Linlin
,
ZHAO Jianxin
. Bifidobacterium longum CCFM1240 relieves constipation caused by downregulation of ERβ by modulating enteric nervous-immune interaction[J]. Food and Fermentation Industries, 2025
, 51(11)
: 1
-11
.
DOI: 10.13995/j.cnki.11-1802/ts.039787
[1] BHARUCHA A E, LACY B E.Mechanisms, evaluation, and management of chronic constipation[J].Gastroenterology, 2020, 158(5):1232-1249.
[2] VRIESMAN M H, KOPPEN I J N, CAMILLERI M, et al.Management of functional constipation in children and adults[J].Nature Reviews.Gastroenterology & Hepatology, 2020, 17(1):21-39.
[3] CHITKARA D K, TALLEY N J, RICHARD L G, et al.Medical presentation of constipation from childhood to early adulthood:A population-based cohort study[J].Clinical Gastroenterology and Hepatology, 2007, 5(9):1059-1064.
[4] PAN R L, WANG L L, XU X P, et al.Crosstalk between the gut microbiome and colonic motility in chronic constipation:Potential mechanisms and microbiota modulation[J].Nutrients, 2022, 14(18):3704.
[5] KHUITUAN P, HUIPAO N, JEANMARD N, et al.Sargassum plagiophyllum extract enhances colonic functions and modulates gut microbiota in constipated mice[J].Nutrients, 2022, 14(3):496.
[6] TANG T, WANG J, JIANG Y Y, et al.Bifidobacterium lactis TY-S01 prevents loperamide-induced constipation by modulating gut microbiota and its metabolites in mice[J].Frontiers in Nutrition, 2022, 9:890314.
[7] 曹永强, 张健, 郑喆, 等.副干酪乳杆菌N1115发酵乳对小鼠便秘的改善作用[J].食品科学, 2018, 39(1):185-191.
CAO Y Q, ZHANG J, ZHENG Z, et al.Therapeutic effect of Lactobacillus paracasei N1115 fermented milk in constipated mice[J].Food Science, 2018, 39(1):185-191.
[8] 梅春霞, 王嘉良, 唐楠, 等.应用雌激素受体β抑制剂建立肠道屏障受损的便秘动物模型[J].食品与发酵工业, 2024, 50(5):35-41.
MEI C X, WANG J L, TANG N, et al.Establishment of constipation animal model with impaired intestinal barrier by estrogen receptor β inhibitors[J].Food and Fermentation Industries, 2024, 50(5):35-41.
[9] 毛丙永. 功能性低聚糖对肠道细菌的影响及机制[D].无锡:江南大学, 2015.
MAO B Y.Effect and mechanism of functional oligosaccharides on intestinal bacteria [D].Wuxi:Jiangnan University, 2015.
[10] LI Y, LI Y R, JIN Y, et al.Involvement of enteric glial cells in colonic motility in a rat model of irritable bowel syndrome with predominant diarrhea[J].Journal of Chemical Neuroanatomy, 2023, 128:102235.
[11] 张程程. 中国不同地区人群肠道中长双歧杆菌的生理特性及肠道调节功能分析[D].无锡:江南大学, 2021.
ZHANG C C.Analysis of physiological characteristics and intestinal regulation function of Bifidobacterium longum in different areas of China[D].Wuxi:Jiangnan University, 2021.
[12] LI B, LI M, LUO Y N, et al.Engineered 5-HT producing gut probiotic improves gastrointestinal motility and behavior disorder[J].Frontiers in Cellular and Infection Microbiology, 2022, 12:1013952.
[13] JIN B, HA S E, WEI L, et al.Colonic motility is improved by the activation of 5-HT2B receptors on interstitial cells of Cajal in diabetic mice[J].Gastroenterology, 2021, 161(2):608-622.
[14] LI T J, YU B P, DONG W G, et al.Ovarian hormone modulates 5-hydroxytryptamine 3 receptors mRNA expression in rat colon with restraint stress-induced bowel dysfunction[J].World Journal of Gastroenterology, 2004, 10(18):2723-2726.
[15] KASHYAP P C, MARCOBAL A, URSELL L K, et al.Complex interactions among diet, gastrointestinal transit, and gut microbiota in humanized mice[J].Gastroenterology, 2013, 144(5):967-977.
[16] FUNG T C, VUONG H E, LUNA C D G, et al.Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut[J].Nature Microbiology, 2019, 4(12):2064-2073.
[17] SCHLUTER J, PELED J U, TAYLOR B P, et al.The gut microbiota is associated with immune cell dynamics in humans[J].Nature, 2020, 588(7837):303-307.
[18] XIONG Y, ZHONG Q, PALMER T, et al.Estradiol resolves pneumonia via ERβ in regulatory T cells[J].JCI Insight, 2021, 6(3):e133251.
[19] LIU W X, LUO X L, TANG J, et al.A bridge for short-chain fatty acids to affect inflammatory bowel disease, type 1 diabetes, and non-alcoholic fatty liver disease positively:By changing gut barrier[J].European Journal of Nutrition, 2021, 60(5):2317-2330.
[20] DEUTSCHER J, FRANCKE C, POSTMA P W.How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria[J].Microbiology and Molecular Biology Reviews, 2006, 70(4):939-1031.
[21] ZHENG Z P, TANG J Y, HU Y N, et al.Role of gut microbiota-derived signals in the regulation of gastrointestinal motility[J].Frontiers in Medicine, 2022, 9:961703.
[22] 姜继鹏, 孙亚楠, 张晨晨, 等.非核糖体肽的生物合成研究进展[J].中国生物工程杂志, 2023, 43(8):86-99.
JIANG J P, SUN Y N, ZHANG C C, et al.Advances in the biosynthesis of nonribosomal peptide[J].China Biotechnology, 2023, 43(8):86-99.
