Food and Fermentation Industries >

2025 , Vol. 51 >Issue 11: 37 - 46

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041944

Atmospheric room temperature plasma mutagenesis and medium optimization of monensin production strain for efficient production

  • ZHANG Shanfei ,
  • LI Xinying ,
  • TIAN Dandan ,
  • ZHANG Xianyuan ,
  • ZHANG Yan ,
  • WANG Dandan ,
  • SONG Shangxia ,
  • SUN Fubao
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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China)
    3(Shandong Shengli Bioengineering Co. Ltd. , Jining 272000, China)

Received date: 2024-12-24

  Revised date: 2025-02-13

  Online published: 2025-06-19

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Abstract

Monensin, a polyether ionophore antibiotic, is extensively utilized across agricultural and pharmaceutical sectors.Streptomyces cinnamonensis, a key microorganism for monensin production, could potentially enhance monensin fermentation yields through strain breeding and selective addition of precursors.This study employed atmospheric room temperature plasma mutagenesis on an industrial monensin-producing strain 2110, successfully generating a high-yield mutant strain with a monensin titer of 7.56 g/L, marking a 10% increase.Further optimization of the fermentation medium for this mutant strain through single-factor and response surface methodology identified the primary components as 35.0 g/L glucose, 41.81 g/L soybean oil, 30.0 g/L soybean meal, 1.0 g/L sodium propionate, 0.25 g/L lysine, 0.12 g/L isoleucine, and 0.16 g/L valine, with soybean oil, isoleucine, and valine significantly impacting monensin production.Under these conditions, fed-batch fermentation in a 5 L bioreactor achieved a yield of 12.23 g/L, representing a 56.56% improvement.These findings offer valuable insights for the industrial production of monensin and demonstrate significant potential for industrial application.

Key words： monensin; atmospheric room temperature plasma; Streptomyces cinnamonensis; mutagenic selection; response surface optimization

Cite this article

ZHANG Shanfei , LI Xinying , TIAN Dandan , ZHANG Xianyuan , ZHANG Yan , WANG Dandan , SONG Shangxia , SUN Fubao . Atmospheric room temperature plasma mutagenesis and medium optimization of monensin production strain for efficient production[J]. Food and Fermentation Industries, 2025 , 51(11) : 37 -46 . DOI: 10.13995/j.cnki.11-1802/ts.041944

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