Food and Fermentation Industries >

2025 , Vol. 51 >Issue 11: 47 - 54

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040161

Optimization of liquid fermentation medium of Sanghuangporus and screening of space mutation strains

  • TIAN Yi ,
  • GAO Jinyun ,
  • XIAO Yuling ,
  • FAN Suqin ,
  • ZHAO Yuke ,
  • MO Zhuanlin ,
  • MO Meihua
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  • 1(School of Food Science, South China Agricultural University, Guangzhou 510000, China)
    2(Basic Experiment and Practice Training Center, South China Agricultural University, Guangzhou 510642, China)

Received date: 2024-06-12

  Revised date: 2024-07-17

  Online published: 2025-06-19

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Abstract

Taking Sanghuangporus as the starting strain (SH-0), the “Long March 5B” carrier rocket was carried out space mutagenesis, and 30 mutant strains were obtained through liquid fermentation screening, after identification,there were 2 Poria, and the rest were Sanghuangporus spp.Taking the dry weight of SH-0 mycelium as the index, the formula of liquid fermentation medium was optimized by uniform design method.The results showed that the optimal formula of liquid fermentation medium of Sanghuangporus was 46.47 g Corn flour, 55.00 g glucose, 22.00 g peptone, 21.94 g soybean flour, 0.07 g potassium dihydrogen phosphate, 36.34 g bran, 0.01 g sodium chloride, 0.29 g magnesium sulfate in 1 l medium.The optimal liquid fermentation medium was used to screen 12 space mutagenic strains of Sanghuangporus and 1 commercially available Sanghuangporus (SH-S) whose mycelial dry weight was significantly greater than that of sh-0.Through multi-dimensional comprehensive comparison of the rescreening strains, the space mutagenic strain sh-68 of Sanghuangporus was finally screened, and its mycelial dry weight was 24.4 g/L;the crude polysaccharide content was 32.99 mg/g;total flavonoids 8.90 mg/g, the total polyphenol content was 7.76 mg/g, and the total antioxidant capacity was 53.9 μmol/g, The clearance rate of DPPH was 91.8%.After giving the corresponding weights to each index, its comprehensive score was 32.46, which was significantly improved on the basis of 22.56 points of the starting strain.

Key words： Sanghuangporus; liquid fermentation; formula optimization; space-flight mutation; strain screening

Cite this article

TIAN Yi , GAO Jinyun , XIAO Yuling , FAN Suqin , ZHAO Yuke , MO Zhuanlin , MO Meihua . Optimization of liquid fermentation medium of Sanghuangporus and screening of space mutation strains[J]. Food and Fermentation Industries, 2025 , 51(11) : 47 -54 . DOI: 10.13995/j.cnki.11-1802/ts.040161

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