Food and Fermentation Industries >

2025 , Vol. 51 >Issue 11: 171 - 178

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041962

Isolation, identification, and screening of probiotic strains with cholesterol-lowering potential from fermented mare’s milk

  • KUERDAN Hudayibieergen ,
  • LIU Xuelian ,
  • KAWUSAER Qiayimaerdan ,
  • HE Yao ,
  • BAHEBAN Yeerjiang ,
  • XINHUA Nabi
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumchi 830017, China)
    2(Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumchi 830017, China)

Received date: 2024-12-26

  Revised date: 2025-01-22

  Online published: 2025-06-19

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Abstract

This study investigates the probiotic properties and cholesterol-lowering abilities of fermentation bacteria, by isolating and purifying four strains of lactic acid bacteria from traditional fermented mare’s milk.The morphological, physiological, and biochemical characteristics of these strains were assessed, along with their growth curves, tolerance to bile salts and artificial gastric fluid, and susceptibility to antibiotics.Additionally, qualitative assays were conducted for bile salt hydrolase activity and cholesterol oxidase to evaluate their cholesterol scavenging potential.The strains were ultimately identified using 16S rRNA gene sequencing.The findings reveal that the isolated strains exhibited strong tolerance to high concentrations of bile salts (3.0 g/L) and artificial gastric fluid (pH 2.0) while remaining sensitive to the majority of the eight antibiotics tested.Distinct white precipitation zones around the filter paper disks of each strain indicated the presence of bile salt hydrolase activity.The cholesterol scavenging rates for single and composite strains in the culture medium ranged from (76.46±1.26)% to (87.96±0.43)%, with the composite strain demonstrating superior capacity for cholesterol compared to individual strains.These strains had been identified as Lactiplantibacillus plantarum.This study suggests that the four isolated strains possess fundamental probiotic attributes and a high safety profile, along with notable cholesterol-lowering capabilities in vitro, which warrant further explored in vivo.

Key words： traditional fermented mare’s milk; probiotic properties; antibiotic sensitivity; bile salt hydrolase; cholesterol-lowering effect

Cite this article

KUERDAN Hudayibieergen , LIU Xuelian , KAWUSAER Qiayimaerdan , HE Yao , BAHEBAN Yeerjiang , XINHUA Nabi . Isolation, identification, and screening of probiotic strains with cholesterol-lowering potential from fermented mare’s milk[J]. Food and Fermentation Industries, 2025 , 51(11) : 171 -178 . DOI: 10.13995/j.cnki.11-1802/ts.041962

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