Isothermal nucleic acid amplification technology

  • GUO Yumei ,
  • JIA Zhenjun ,
  • LIU Rui
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  • (School of Investigation, People’s Public Security University of China, Beijing 100038, China)

Received date: 2024-12-12

  Revised date: 2025-02-10

  Online published: 2025-06-19

Abstract

As global food safety issues become more severe, the development of rapid and sensitive detection technologies has become increasingly critical.Characterized by efficiency, speed, and simplicity, nucleic acid isothermal amplification technologies are playing an increasingly vital role in the field of food safety detection.This paper systematically explores the applications of various nucleic acid isothermal amplification methods in food safety detection, including loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), enzymatic recombinase amplification (ERA), strand displacement amplification (SDA), and nucleic acid multi-arm primers and circular optimization technologies.The discussion encompasses the main types, basic principles, specific applications, advantages of combining these methods with other technologies, and future development trends.By analyzing recent research advancements, this study aims to provide new perspectives and references in the field of food safety detection.It further seeks to promote the development and application of these emerging technologies.

Cite this article

GUO Yumei , JIA Zhenjun , LIU Rui . Isothermal nucleic acid amplification technology[J]. Food and Fermentation Industries, 2025 , 51(11) : 435 -448 . DOI: 10.13995/j.cnki.11-1802/ts.041839

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