Food and Fermentation Industries >

2025 , Vol. 51 >Issue 11: 55 - 64

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040144

Isolation and identification of passion fruit derived pathogenic bacteria and preliminary study on inhibitory activity of bactericidal agents

  • YANG Dingzhao ,
  • SHUAI Liang ,
  • TANG Suizhi ,
  • YIN Feilong ,
  • HE Meiying ,
  • SONG Mubo ,
  • LIU Chunyou ,
  • LIU Yunfen
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  • 1(College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China)
    2(Guangxi Key Laboratory of Health Care Food Science and Technology, Hezhou 542899, China)
    3(School of Chemistry and Food Science, Nanchang Normal University, Nanchang 330032, China)

Received date: 2024-06-13

  Revised date: 2024-07-05

  Online published: 2025-06-19

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Abstract

This study aimed to clarify the causative organisms of diseases of postharvest passion fruit and the inhibitory activity of plant-derived bacteriostatic agents against the main pathogenic bacteria, providing a reference for the disease control of postharvest passion fruit.This study used the tissue isolation method to isolate and purify the pathogenic bacteria from naturally infected passion fruit, identified the pathogenic bacteria by morphological observation and biological analysis, screened for inhibitors with inhibitory effects on the pathogenic bacteria by using the mycelial growth rate method, and further investigated the inhibitory virulence of phytic acid, citral, and phenyl-lactic acid and their effects on malondialdehyde content, cell membrane integrity, nucleic acid and protein leakage of the primary pathogen.Results showed that the pathogenic bacteria causing postharvest passion fruit diseases were Fusarium, Colletotrichum, Alternaria, and Meyerozyma.Based on the pathogenicity test, Fusarium oxysporum was determined to be the primary pathogen.Phytic acid, citral, and benzyl lactic acid could significantly inhibit the mycelial growth and spore germination of Fusarium oxysporum and be able to destroy the integrity of the cell membrane.The EC50 of phytic acid, citral, and benzyl lactic acid was 0.082 4%, 0.293 9 μL/mL, and 2.277 0 g/L, respectively.The germination rates of 0.2% phytic acid, 0.8 μL/mL citral, and 5 g/L benzyl lactic acid were only 14.91%, 21.03%, and 20.32%, and propidium iodide staining rate was 66.85%, 84.73%, 84.46%.Moreover, studies showed that phytic acid, citral, and phenyl lactic acid treatments significantly increased nucleic acid and protein leakage and malondialdehyde content, with the concentration-dependent.All the above results indicate that phytic acid, citral, and benzyl lactic acid destroy the structure of the cell membrane of F. oxysporum, thus affecting the normal growth of the mycelium to achieve a better bacterial inhibition effect, which provides the theoretical foundation for the application in food preservation.

Key words： postharvest passion fruit; pathogen identification; bacteriostatic agent; toxicity measurement

Cite this article

YANG Dingzhao , SHUAI Liang , TANG Suizhi , YIN Feilong , HE Meiying , SONG Mubo , LIU Chunyou , LIU Yunfen . Isolation and identification of passion fruit derived pathogenic bacteria and preliminary study on inhibitory activity of bactericidal agents[J]. Food and Fermentation Industries, 2025 , 51(11) : 55 -64 . DOI: 10.13995/j.cnki.11-1802/ts.040144

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