Food and Fermentation Industries >

2025 , Vol. 51 >Issue 12: 36 - 43

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040348

Antibacterial mechanism of Litsea cubeba extract against Escherichia coli and its application in fish preservation

  • LIU Menglong ,
  • CHEN Cong ,
  • LU Kaixiang ,
  • WEI Meijuan ,
  • HU Yongjin ,
  • XUE Qiaoli
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  • 1(College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China)
    2(School of Public Health, Dali University, Dali 671000, China)
    3(Editorial Department of Journal of Yunnan Agricultural University, Yunnan Agricultural University, Kunming 650201, China)

Received date: 2024-06-30

  Revised date: 2024-08-21

  Online published: 2025-07-11

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Abstract

This study aimed to elucidate the antibacterial mechanism of Litsea cubeba ethanol extract (LCEE) and explore its potential application in food preservation.This investigation involved a comprehensive analysis of the antibacterial activity of LCEE, focusing on its effects on cell wall-membrane damage, decrease in energy substances, and morphological changes observed through electron microscopy, using Escherichia coli as the model organism.Additionally, the effectiveness of LCEE in fish preservation was assessed through experimental trials.Results revealed that LCEE possessed significant antibacterial activity against a variety of foodborne pathogens.LCEE caused continuous leakage of intracellular ions, proteins, nucleic acids, and alkaline phosphatase by damaging the bacterial cell walls and membranes, and caused a significant decrease in intracellular ATP levels.The surface of cell treated with LCEE showed wrinkles collapses and holes, and the leakage of cell contents led to the emergence of a large number of intracellular hollow cavities.Furthermore, the use of LCEE has been shown to enhance the water holding capacity of fish during storage, delay discoloration, and control the increase of total volatile basic nitrogen levels, total colony counts, and coliform counts, as well as extend the shelf life of fish for more than 2 days.In conclusion, the discovery of LCEE has further enriched the antimicrobial constituents of Litsea cubeba fruits, which inhibit the growth of Escherichia coli through multiple mechanisms, with promising results in food preservation.

Key words： Litsea cubeba ethanol extract; Escherichia coli; antibacterial mechanism; cell wall-membrane damage; fish preservation

Cite this article

LIU Menglong , CHEN Cong , LU Kaixiang , WEI Meijuan , HU Yongjin , XUE Qiaoli . Antibacterial mechanism of Litsea cubeba extract against Escherichia coli and its application in fish preservation[J]. Food and Fermentation Industries, 2025 , 51(12) : 36 -43 . DOI: 10.13995/j.cnki.11-1802/ts.040348

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