To investigate the anti-inflammatory activity of pH-responsive polyphenol delivery systems, zein-phlorotannin (PTN)-carbon quantum dots (CQDs) nanoparticles were constructed by loading PTN into zein-CQDs nanoparticles.Zein-PTN-CQDs-FeⅢ nanoparticles were further prepared by adding tannic acid and Fe3+ based on zein-PTN-CQDs nanoparticles.The pH-responsive release characteristics and anti-inflammatory activity of the nanoparticles were explored through experiments on polyphenol release in vitro, cell toxicity, cell apoptosis imaging, anti-inflammatory properties, and reactive oxygen species (ROS) detection.Results showed that the addition of CQDs effectively reduced the particle size and increased the encapsulation efficiency.pH-responsive polyphenol-metal coating networks were formed on the surface of zein-PTN-CQDs nanoparticles through the combination of tannic acid and Fe3+.The release rates of PTN-CQDs-FeIII nanoparticles under acidic conditions of pH 6.2, 5, and 4 were 78%, 89%, and 96%, respectively, significantly higher than those under neutral conditions (28%), exhibiting sustained release and pH-responsive characteristics.For lipopolysaccharide-stimulated RAW 264.7 cells, zein-PTN-CQDs-FeⅢ nanoparticles significantly reduced ROS production and exhibited superior inhibition of pro-inflammatory factors (IL-1β, IL-6, and TNF-α) and promotion of anti-inflammatory factors (IL-10 and prostaglandin E2) compared to the PTN group and zein-CQDs-FeⅢ group.
GAN Miaoyu
,
ZANG Yunfang
,
ZENG Fanzheng
,
CAO Ailing
,
LIANG Yan
,
LIU Quansheng
,
CAI Luyun
,
LI Yujin
. Anti-inflammatory activity study of pH-responsive metal-polyphenol structure-coated nanoparticles[J]. Food and Fermentation Industries, 2025
, 51(12)
: 92
-99
.
DOI: 10.13995/j.cnki.11-1802/ts.039486
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