Food and Fermentation Industries >

2025 , Vol. 51 >Issue 12: 115 - 122

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039965

Atmospheric room temperature plasma mutagenesis breeding and fermentation optimization of Aspergillus niger with high yield of tannase

  • MA Dongxu ,
  • LI Zhonghui ,
  • ZHANG Ziheng ,
  • WANG Xin ,
  • GONG Dachun ,
  • SHI Xiaotao
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  • 1(College of Hydraulic & Environmental Engineering, China Three Gorges University, Yichang 443002, China)
    2(Key Laboratory of Functional Yeast of China Light Industry, China Three Gorges University, Yichang 443002, China)
    3(Hubei Engineering Research Center for Biological Jiaosu, China Three Gorges University, Yichang 443002, China)

Received date: 2024-05-22

  Revised date: 2024-09-07

  Online published: 2025-07-11

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Abstract

Tannase is a biocatalyst with high performance for hydrolyzing tannin substances.It has been widely used in various industries such as food, beverage, and medicine.As an important microorganism for producing tannase, Aspergillus niger needs to further increase enzyme production through the breeding of high-yield strain and the optimization of fermentation process to meet the growing demand for industrial applications.In this study, the optimal conditions for atmospheric room temperature plasma (ARTP) mutagenesis in original strain Aspergillus niger LH were 3×106 CFU/mL spore concentration, 100 W output power, 10 L/min flow rate of the carrier gas, 2 mm distance, and 240 s exposure time.Plate colorimetric analysis revealed that 49 mutants displayed large color-changing circle.According to shake flask fermentation, mutant LH2301 possessed the highest enzyme activity and stable enzyme production.The tannase activity reached 2.292 U/mL, 2.27 times higher than the original strain.Through orthogonal experiments, the optimal cultivation conditions were 5 g/L glucose, 21 g/L yeast extract powder, 4.0 initial pH, 3.5% inoculation amount, and 20 g/L tannic acid.The enzyme activity of the mutant strain could be increased to 6.105 U/mL.Finally, the tannase produced from the mutant strain was investigated.Its molecular weight was 90 kDa.When the temperature was at 40-50 ℃ and pH 3.0-5.0, the enzyme exhibited well catalytic activity.The optimal temperature for this enzyme was 50 ℃ and the optimal pH was 4.0, metal ions Ca2+, K+, and Al3+ played a role in promoting enzyme activity.Our research expands the enzyme library resources of tannase and provides technical support for industrial application.

Key words： Aspergillus niger; tannase; atmospheric room temperature plasma mutagenesis; fermentation; enzymatic properties

Cite this article

MA Dongxu , LI Zhonghui , ZHANG Ziheng , WANG Xin , GONG Dachun , SHI Xiaotao . Atmospheric room temperature plasma mutagenesis breeding and fermentation optimization of Aspergillus niger with high yield of tannase[J]. Food and Fermentation Industries, 2025 , 51(12) : 115 -122 . DOI: 10.13995/j.cnki.11-1802/ts.039965

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