Food and Fermentation Industries >

2025 , Vol. 51 >Issue 12: 123 - 131

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040833

Metabolomic analysis of effects of camel milk on anti-inflammatory and immune functions in mice

  • LIU Shibo ,
  • YAO Xinkui ,
  • MENG Jun ,
  • ZENG Yaqi ,
  • SUN Zhiwen ,
  • WANG Jianwen
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  • 1(College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China)
    2(Xinjiang Key Laboratory of Horse Breeding and Exercise Physiology, Urumqi 830052, China)

Received date: 2024-08-22

  Revised date: 2024-09-19

  Online published: 2025-07-11

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Abstract

This study investigated the effects of camel milk on the anti-inflammatory and immune functions of mice through plasma metabolomics and enzyme-linked immunosorbent assay (ELISA) analysis.A total of 32 ICR mice were randomly divided into two groups,including a camel milk group and a control group.The mice were orally administered 10 mL/kg body weight of camel milk or distilled water daily for 28 days.After the administration period, liver and blood samples were collected.Inflammatory markers in the mice were measured using ELISA, and non-targeted metabolomics of serum metabolites was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by analysis of the results.Results demonstrated that camel milk administration elevated the levels of interleukin 6 (IL-6), secretory immunoglobulin A (sIgA), interleukin 1β (IL-1β), and interferon γ (IFN-γ), while reducing tumor necrosis factor-α (TNF-α) levels in the liver of mice.A total of 207 differential metabolites were identified between the two groups, with 201 metabolites significantly upregulated and 6 significantly downregulated in the control group.Among these differential metabolites, lipid metabolites such as lysophosphatidylcholine (LPC) accounted for the majority, and these metabolites were significantly enriched in pathways including pyrimidine metabolism and the tricarboxylic acid (TCA) cycle.These findings suggest that camel milk may exert its anti-inflammatory effects in mice by modulating lipid metabolism, pyrimidine metabolism, and the TCA cycle.The results provide valuable data for the functional development and utilization of camel milk.

Key words： camel milk; metabolomics; anti-inflammatory; mice

Cite this article

LIU Shibo , YAO Xinkui , MENG Jun , ZENG Yaqi , SUN Zhiwen , WANG Jianwen . Metabolomic analysis of effects of camel milk on anti-inflammatory and immune functions in mice[J]. Food and Fermentation Industries, 2025 , 51(12) : 123 -131 . DOI: 10.13995/j.cnki.11-1802/ts.040833

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