Effect of Lactobacillus paracasei and Kluyveromyces marxianus on quality of Tibetan pig ham

  • WANG Fei ,
  • Luosangyangji ,
  • HUANG Jingjing ,
  • LUO Zhang ,
  • LIU Zhendong ,
  • ZHANG Erhao ,
  • Lamaocaidan ,
  • LI Liang ,
  • XIE Ningning
Expand
  • 1(School of Food Science, Xizang Agriculture and Animal Husbandry College, Nyingchi 860000,China)
    2(Institute of Agricultural Products Processing, Anhui Academy of Agricultural Sciences, Hefei 230031,China)
    3(Institute of Food and Drug Inspection of Tibet Autonomous Region, Lhasa 850000,China)

Received date: 2024-08-13

  Revised date: 2024-09-03

  Online published: 2025-07-11

Abstract

As a characteristic economic pig breed in the plateau, Tibetan pigs have delicious meat and rich nutrition, making them an excellent fermented meat raw material.Using Lactobacillus paracasei (LP) and Kluyveromyces marxianus (KM) to inoculate fermented Tibetan pig hind legs, this study investigated the changes in physicochemical properties (pH value, water activity) and physical properties (color difference, texture characteristics) of cured, air dried, fermented, and matured Tibetan pig ham under single strain (LP or KM) and complex strain (LP+KM, abbreviated as LK) methods, and compared the differences in volatile flavor and sensory evaluation of Tibetan pig ham at the fermentation endpoint.Results showed that compared with natural fermentation (NF, unvaccinated), inoculation fermentation showed significant differences (P<0.05) after fermentation and maturation.Inoculation fermentation reduced the pH value (below 6.75), water activity (decreased by 2%-10%), hardness, chewiness, and adhesiveness of Tibetan pig ham, while improving its color, brightness, and elasticity (P<0.05).Among them, the elasticity of LP and LK fermentation increased by about 40%.Inoculation fermentation increased the total aldehyde, total ketone, and total ester contents of mature ham, and the KM and LK treatment groups formed various new alcohol, aldehyde, and ketone flavor compounds.In addition, the sensory qualities such as tissue morphology, color, appearance, and taste of the LK treatment group were superior to those of the single bacterium fermentation group.It can be seen that the combination of LP and KM plays an important role in enhancing the quality and flavor of Tibetan pig ham, and can be used as a quality improver in the industrial production of Tibetan pig ham.

Cite this article

WANG Fei , Luosangyangji , HUANG Jingjing , LUO Zhang , LIU Zhendong , ZHANG Erhao , Lamaocaidan , LI Liang , XIE Ningning . Effect of Lactobacillus paracasei and Kluyveromyces marxianus on quality of Tibetan pig ham[J]. Food and Fermentation Industries, 2025 , 51(12) : 249 -258 . DOI: 10.13995/j.cnki.11-1802/ts.040734

References

[1] ZHAO L Y, ERASMUS S, YANG P, et al. Establishing the relations of characteristic aroma precursors and volatile compounds for authenticating Tibetan pork[J]. Food Chemistry, 2023, 427:136717.
[2] ZHOU G H, ZHAO G M. Biochemical changes during processing of traditional Jinhua ham[J]. Meat Science, 2007, 77(1):114-120.
[3] LI G P, LI S M, WEN Y L, et al. Correlation between the characteristic flavour and microbial community of Xuanwei ham after ripening[J]. Fermentation, 2024, 10(8):392.
[4] 李丹丹, 蒲丽, 罗章. 西藏主要产地帕宫营养成分分析与评价[J]. 高原农业, 2020, 4(2):178-184.
LI D D, PU L, LUO Z. Nutritional analysis and evaluation of pagong from major production areas in Tibet[J]. Journal of Plateau Agriculture, 2020, 4(2):178-184.
[5] BARCENILLA C, DUCIC M, LÓPEZ M, et al. Application of lactic acid bacteria for the biopreservation of meat products: A systematic review[J]. Meat Science, 2022, 183:108661.
[6] GE Q F, PEI H J, LIU R, et al. Effects of Lactobacillus plantarum NJAU-01 from Jinhua ham on the quality of dry-cured fermented sausage[J]. LWT, 2019, 101:513-518.
[7] GONG X H, MI R F, CHEN X, et al. Evaluation and selection of yeasts as potential aroma enhancers for the production of dry-cured ham[J]. Food Science and Human Wellness, 2023, 12(1):324-335.
[8] GOGOI P, BORPUZARI R N, BORPUZARI T, et al. Effect of starter cultures and packaging methods on amino acid profile and eating quality characteristics of pork ham[J]. Journal of Food Science and Technology, 2015, 52(8):5329-5334.
[9] 鄢嫣, 杨明柳, 周迎芹, 等. 不同发酵剂对发酵牛肉品质、风味特性和安全性的影响[J]. 中国食品学报, 2024, 24(4):170-185.
YAN Y, YANG M L, ZHOU Y Q, et al. Effects of different starter cultures on the quality, flavor characteristics and safety of fermented beef[J]. Journal of Chinese Institute of Food Science and Technology, 2024, 24(4):170-185.
[10] TIAN Z Q, ZHU Q J, CHEN Y S, et al. Studies on flavor compounds and free amino acid dynamic characteristics of fermented pork loin ham with a complex starter[J]. Foods, 2022, 11(10):1501.
[11] CEBRIÁN E, RODRÍGUEZ M, PEROMINGO B, et al. Efficacy of the combined protective cultures of Penicillium chrysogenum and Debaryomyces hansenii for the control of ochratoxin a hazard in dry-cured ham[J]. Toxins, 2019, 11(12):710.
[12] 史巧, 李聪, 王桂瑛, 等. 不同加工年份诺邓火腿理化特性研究[J]. 食品研究与开发, 2023, 44(8):46-52.
SHI Q, LI C, WANG G Y, et al. Study on the physicochemical characteristics of Nuodeng ham with different processing times[J]. Food Research and Development, 2023, 44(8):46-52.
[13] ANDRÉS A I, CAVA R, VENTANAS J, et al. Sensory characteristics of Iberian ham: Influence of salt content and processing conditions[J]. Meat Science, 2004, 68(1):45-51.
[14] REN H Y, DENG Y P, WANG X H. Effect of a compound starter cultures inoculation on bacterial profile and biogenic amine accumulation in Chinese Sichuan sausages[J]. Food Science and Human Wellness, 2022, 11(2):341-348.
[15] DENG J Y, XU H, LI X M, et al. Correlation of characteristic flavor and microbial community in Jinhua ham during the post-ripening stage[J]. LWT, 2022, 171:114067.
[16] 王德宝, 赵丽华, 田建军, 等. 不同发酵剂对发酵香肠中风味物质释放及有害生物胺控制的影响[J]. 中国食品学报, 2019, 19(8):89-96.
WANG D B, ZHAO L H, TIAN J J, et al. Effects of different starter cultures on the flavor release and control of the harmful biogenic amines in the fermented mutton sausages[J]. Journal of Chinese Institute of Food Science and Technology, 2019, 19(8):89-96.
[18] YANG L, LI H J, WU H, et al. Staphylococcus inoculation enhances the sensorial attributes of Chinese bacon by coordinating the composition of flavor compounds through amino acid metabolism[J]. Food Research International, 2024, 178:113936.
[19] PEREA-SANZ L, LÓPEZ-DÍEZ J J, BELLOCH C, et al. Counteracting the effect of reducing nitrate/nitrite levels on dry fermented sausage aroma by Debaryomyces hansenii inoculation[J]. Meat Science, 2020, 164:108103.
[20] 黄露, 郇延军. 抗氧化型发酵剂对香肠发酵过程中脂肪氧化的影响[J]. 食品与发酵工业, 2016, 42(12):38-44.
HUANG L, HUAN Y J. Effect of antioxidant starter cultures on lipid oxidation of sausage during fermentation[J]. Food and Fermentation Industries, 2016, 42(12):38-44.
[21] ZHANG J, ZHAO K, LI H H, et al. Physicochemical property, volatile flavor quality, and microbial community composition of Jinhua fatty ham and lean ham: A comparative study[J]. Frontiers in Microbiology, 2023, 14:1124770.
[22] 张倩, 张新斌, 李君冉, 等. 发酵肉制品中乳酸菌抗氧化作用及其对感官品质影响的研究进展[J]. 农产品加工, 2024(5):83-87; 92.
ZHANG Q, ZHANG X B, LI J R, et al. Research progress on the antioxidant effect of lactic acid bacteria in fermented meat products and its effect on sensory quality[J]. Farm Products Processing, 2024(5):83-87; 92.
[23] 兰沁洁, 宋杰, 陈沫, 等. 乳酸菌和酵母菌复配发酵对羊肉香肠食用品质的影响[J]. 食品与发酵工业, 2024, 50(6):24-33.
LAN Q J, SONG J, CHEN M, et al. Effect of compound fermentation of lactic acid bacteria and yeast on eating quality of mutton sausages[J]. Food and Fermentation Industries, 2024, 50(6):24-33.
[24] 陈俊宏, 王洋, 李芹, 等. 乳酸菌发酵工艺对肉糜理化性质及品质的影响[J]. 中国调味品, 2021, 46(6):5-10.
CHEN J H, WANG Y, LI Q, et al. Effect of lactic acid bacteria fermentation process on the physicochemical properties and quality of minced meat[J]. China Condiment, 2021, 46(6):5-10.
[25] LI C, ZHENG Z J, WANG G Y, et al. Revealing the intrinsic relationship between microbial communities and physicochemical properties during ripening of Xuanwei ham[J]. Food Research International, 2024, 186:114377.
[26] LI X W, LYU S, SHI T T, et al. Exopolysaccharides from yoghurt fermented by Lactobacillus paracasei: Production, purification and its binding to sodium caseinate[J]. Food Hydrocolloids, 2020, 102:105635.
[27] 母雨, 苏伟, 母应春. 盘县火腿深度腐败的微生物及挥发性风味化合物表征[J]. 食品科学, 2021, 42(8):221-228.
MU Y, SU W, MU Y C. Characterization of microbial community and volatile flavor compounds of deep spoiled Panxian dry-cured ham[J]. Food Science, 2021, 42(8):221-228.
[28] 李钰芳, 施娅楠, 李祥, 等. 传统工艺下发酵时间对大河乌猪火腿挥发性风味物质的影响[J]. 食品与发酵工业, 2022, 48(6):160-167.
LI Y F, SHI Y N, LI X, et al. Effect of fermentation time on volatile flavor compounds of Dahe black pig ham during traditional processing[J]. Food and Fermentation Industries, 2022, 48(6):160-167.
[29] ZHENG Z J, TIAN M, LIAO G Z, et al. Evaluation of the effects of compound curing agents on the lipid profiles and volatile flavors in Nuodeng ham based on lipidomics and GC-IMS analysis[J]. Food Research International, 2024, 176:113810.
[30] 母雨, 苏伟, 母应春. 盘县火腿微生物多样性及主体挥发性风味解析[J]. 食品研究与开发, 2019, 40(15):77-85.
MU Y, SU W, MU Y C. Analysis of microbial diversity and key volatile flavor compounds of Panxian dry-cured ham[J]. Food Research and Development, 2019, 40(15):77-85.
[31] LI W Q, CHEN Y P, BLANK I, et al. GC×GC-ToF-MS and GC-IMS based volatile profile characterization of the Chinese dry-cured hams from different regions[J]. Food Research International, 2021, 142:110222.
[32] 郑荣美, 张磊, 郑淮升, 等. 不同杀菌方式对白切香猪肉品质和挥发性成分的影响[J]. 食品与发酵工业, 2024, 50(22):246-253.
ZHENG R M, ZHANG L, ZHENG H S, et al. Analysis of differences in quality and volatile components of cooked Xiang pork with different sterilization methods[J]. Food and Fermentation Industries, 2024, 50(22):246-253.
Outlines

/

〈 〉