Food and Fermentation Industries >

2025 , Vol. 51 >Issue 12: 319 - 326

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040667

Rapid determination of ten alkaloids of Gelsemium elegans with dispersed solid phase extraction-high performance liquid chromatographytandem mass spectrometry technique

  • ZENG Xi ,
  • XU Zhenlin ,
  • HU Ling ,
  • MAO Xinwu ,
  • LIU Chunsheng ,
  • LIU Xin ,
  • WANG Yu ,
  • YU Yang
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  • 1(Guangzhou Institute for Food Inspection, Guangzhou 511400, China)
    2(College of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, China)
    3(Huangpu Customs Technology Center, Dongguan 510700, China)

Received date: 2024-08-14

  Revised date: 2024-10-22

  Online published: 2025-07-11

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Abstract

The alkaloids in Gelsemium elegans are the main components leading to acute poison, which threat to the public health seriously.This work established a method for simultaneously analysis of ten alkaloids in G.elegans using dispersed solid phase extraction-high performance liquid chromatography tandem mass spectrometry technique.Food samples were extracted and ultrasound by 1%HCl before the pH was adjusted to 11-12.And then acetonitrile were added to vortex, latter NaCl were added to assisted centrifugation and stratification.The supernatant was dehydrated and purified by Na2SO4 and C18, respectively.After centrifugation, the supernatant was dried by nitrogen and then redissolved by acetonitrile.The sample was conducted in positive electrospray ionization (ESI+) mode under multiple reaction monitoring mode, coupled with gradient elution by 0.1% NH4OH-10 mmol/L CH3COONH4 solvent and acetonitrile as mobile phase and Hilic (2.1 mm×100 mm×1.7 μm) chromatographic column.The quantification of alkaloids were conducted by matrix standard method.As a result, the matrix effect were 13.1%-3 692.7%, which can be eliminated by corresponding matrix standard.The linearity were good in the range of 0.5-10.0 ng/mL, with the regression coefficients (R) all above 0.995.The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.075-0.225 ng/g and 0.250-0.750 ng/g, respectively.The recoveries were 79.3%-118.7%, and the relative standard deviations were 0.1%-12.1% (n=6) in four different sorts of food samples at three spiked levels.This method is easy, effective and high-sensitive, laid the foundation of rapid detection technique of acute G.elegans poison and owned critical significance of guarantee the public health.

Key words： high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS); dispersed solid phase extraction; Gelsemium elegans poisoning; Gelsemium elegans mistaken; graceful jesamine; alkaloids; Gelsemium elegans alkaloids

Cite this article

ZENG Xi , XU Zhenlin , HU Ling , MAO Xinwu , LIU Chunsheng , LIU Xin , WANG Yu , YU Yang . Rapid determination of ten alkaloids of Gelsemium elegans with dispersed solid phase extraction-high performance liquid chromatographytandem mass spectrometry technique[J]. Food and Fermentation Industries, 2025 , 51(12) : 319 -326 . DOI: 10.13995/j.cnki.11-1802/ts.040667

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