Lipase is a biocatalyst with an important role in several biochemical reactions.Bacillus subtilis lipase A (LipA) is one of the lipases with the smallest molecular weight.Due to its strong adaptability to substrate, strong alkaline resistance, no cover structure, and no need for interface activation, it has important application prospects in food, medicine, paper making, detergent, and other aspects.To improve the fermentation level of LipA, the study was expressed in Escherichia coli BL21 (DE3), and a LipA-expressing strain of the anagen promoter ynhG was constructed to increase the expression of LipA by up-regulating the expression of the ynhG promoter in the growth phase.Characterized with enhanced green fluorescent protein (EGFP), saturation mutation in the -10, -35, and RBS of the ynhG promoter to obtain the sequence of the enhanced ynhG promoter with increased initiation intensity.According to the enhanced ynhG promoter sequence, the enhanced ynhG-LipA-BL21 (DE3) expression strain showed that the enzyme yield of the enhanced ynhG promoter increased by 31% to 137% compared with the wild-type ynhG promoter.The research results will provide important basic data for improving the application of Bacillus subtilis lipase in the industrial field.
DU Feifan
,
LI Jingjing
,
ZHENG Lanxin
,
ZHANG Di
,
YE Huimin
,
DENG Junwei
,
QIN Yunan
,
HUANG Qiuyan
,
CAO Shilin
,
ZENG Xinan
. Optimization of lipase expression by anagen-specific promoters[J]. Food and Fermentation Industries, 2025
, 51(14)
: 106
-112
.
DOI: 10.13995/j.cnki.11-1802/ts.039232
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