To further investigate the effect of bioactive peptides on autophagy flux, this study used an inverted microscope and flow cytometry to observe the effects of different types of bioactive peptides on LO2 hepatocytes.Then Lujiu was prepared with bioactive peptides and Baijiu as raw materials, the peptide retention rate was used as an indicator, and the technology of Lujiu was optimized by a single factor experiment and response surface experiment according to light transmittance index.Results showed that cell survival was observed through an inverted microscope, and 5.00 mg/mL was finally selected as the highest concentration for the test of autophagy activity.Corn peptide-1 and corn peptide-3 could promote autophagy in the range of 0.63-5.00 mg/mL, while wheat peptide-1 and wheat peptide-6 could inhibit it in the concentration of 5.00 mg/mL, among which corn peptide-1 had the most obvious effect on autophagy.The best technological conditions of Lujiu were that the degree of base liquor was 48%vol, the flavor type of base liquor was fragrant, the temperature of base liquor was room temperature, the type of bioactive peptide was corn peptide-1, and the additional amount of bioactive peptide was 15.09 mg/mL.Under these conditions, the light transmittance of Lujiu was high and the taste was good.The sensory score was 93.Finally, the mice were administered with normal saline, edible alcohol, base liquor, and peptide liquor, respectively.After being killed, the pathological changes of liver tissues were observed.It was found that Lujiu reduced the degree of liver damage in the experimental mice to a certain extent.
WANG Yaning
,
XUE Jie
,
ZHONG Heng
,
LUO Ruiqi
,
SHI Jun
,
SONG Tao
,
YU Jiajun
,
LI Jumei
,
TANG Yali
,
HUANG Benchen
. High-throughput screening of bioactive peptides based on autophagy and application in Lujiu products[J]. Food and Fermentation Industries, 2025
, 51(14)
: 139
-149
.
DOI: 10.13995/j.cnki.11-1802/ts.040674
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