Food and Fermentation Industries >

2025 , Vol. 51 >Issue 14: 195 - 201

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040442

Effect of environmental stress on ability of Lactiplantibacillus paraplantarum L-ZS9 to synthesize exopolysaccharides

  • YUAN Lu ,
  • SONG Yunlong ,
  • LIU Lei ,
  • YANG Yao ,
  • ZHANG Chengyi ,
  • LI Xiaoli ,
  • LONG Haiqi ,
  • RAO Yu
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  • 1(School of Food and Biological Engineering, Xihua University, Chengdu 610039, China)
    2(School of Animal Medicine and Biosafety, Lanzhou University, Lanzhou 730000, China)

Received date: 2024-07-09

  Revised date: 2024-10-30

  Online published: 2025-08-01

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Abstract

Lactic acid bacteria exopolysaccharides have numerous probiotic functions, such as regulating intestinal flora, regulating immunity, and alleviating lactose intolerance.However, their production faces various environmental stresses in industrial applications and the human gastrointestinal system, including pH, bile salt, low temperature, etc., resulting in low yield and economic benefits of exopolysaccharides.The purpose of this study was to explore the effects of acid, bile salt, low temperature, and ultra-high pressure stress on the ability of lactic acid bacteria to produce exopolysaccharides.Additionally, this study aimed to improve the production of exopolysaccharides by subjecting lactic acid bacteria to various environmental stresses and expanding their applications.Results showed that under acid stress, Lactiplantibacillus paraplantarum L-ZS9 increased its EPS production capacity with decreasing pH.The strain had the strongest EPS production capacity under pH 3.0 acid stress.The EPS production capacity of L-ZS9 was improved under bile salt stress, and the EPS production ability of a single colony was the strongest when the bile salt concentration was 1 g/L bile salt stress.However, both acid and bile salt could promote EPS production by L-ZS9 within certain limits.They also had detrimental effects when exceeding those limits for instance causing strain death under conditions of pH 2.0 or a bile salt concentration of 5 g/L. Under low-temperature stress, there was no significant improvement in the EPS production capacity of L-ZS9 observed.On the other hand, under ultra-high pressure stress, L-ZS9 demonstrated an increase in its EPS production capacity with increasing pressure, with a single colony showing its strongest EPS production at 300 MPa.In conclusion, environmental stress can be beneficial for improving the ability of L-ZS9 to produce exopolysaccharides.

Key words： Lactiplantibacillus paraplantarum; exopolysaccharides; acid stress; bile salt stress; low temperature stress; ultra-high pressure stress

Cite this article

YUAN Lu , SONG Yunlong , LIU Lei , YANG Yao , ZHANG Chengyi , LI Xiaoli , LONG Haiqi , RAO Yu . Effect of environmental stress on ability of Lactiplantibacillus paraplantarum L-ZS9 to synthesize exopolysaccharides[J]. Food and Fermentation Industries, 2025 , 51(14) : 195 -201 . DOI: 10.13995/j.cnki.11-1802/ts.040442

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