Food and Fermentation Industries >

2025 , Vol. 51 >Issue 14: 228 - 235

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042100

Potential mechanism of chaga mushroom in treatment of Alzheimer’s disease based on network pharmacology and molecular docking

  • FU Dandan ,
  • SHAO Xinyuan ,
  • ZHANG Qi ,
  • ZHANG Di ,
  • PENG Xinnuan ,
  • LIU Yanshan ,
  • SUN Jianrui ,
  • GONG Qiang
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  • 1(School of Food and Biological Engineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Food Microbiology Engineering and Technology Research Center, Luoyang 471023, China)
    3(University Key Laboratory of Microbiology Resources Development and Utilization of Henan University of Science and Technology, Luoyang 471023, China)

Received date: 2025-01-10

  Revised date: 2025-03-17

  Online published: 2025-08-01

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Abstract

To investigate the potential mechanisms of Chaga mushroom in the treatment of Alzheimer’s disease (AD), network pharmacology and molecular docking techniques were used to predict the active components, key targets, and signalling pathways.The active components and target genes of Inonotus obliquus were obtained from databases such as TCMSP and literature.The disease targets of AD were acquired through databases such as GeneCards to identify the common targets of Inonotus obliquus and Alzheimer’s disease.The protein-protein interaction (PPI) network diagram of common targets was constructed using the String database, and the interaction network of “active components-key targets” was constructed by Cytoscape software.GO and KEGG enrichment analyses were performed by DAVID and Metascape databases.Molecular docking simulations were conducted using Autodock software.Finally, 9 active components and 3 397 targets of Alzheimer’s disease were screened out.Twenty key targets were obtained by PPI, such as MMP3, IL6, AKT1, IL10, TNF, MAPK1, HSP90AB1, and so on.KEGG pathway analysis showed that Inonotus obliquus played an anti-AD role through the signalling pathways of lipid and atherosclerosis, hepatitis B, human cytomegalovirus infection, interleukin-17, Alzheimer’s disease, and so on.GO enrichment analysis showed that it was related to RNA polymeraseⅡtranscription factor complex, an integral component of the plasma membrane, positive regulation of pri-miRNA transcription from RNA polymeraseⅡpromoter, and so on.20 key targets were docked with their corresponding active components to verify that they could bind stably, which predicted that quercetin played an important role in alleviating Alzheimer’s disease.Chaga mushroom could interfere with multiple signalling pathways, such as lipid and atherosclerosis, as well as through multiple components and targets, providing theoretical support for the development of medicine and food homologous health foods.

Key words： Inonotus obliquus; homology of medicine and food; senile dementia; protein-protein interaction; signalling pathway

Cite this article

FU Dandan , SHAO Xinyuan , ZHANG Qi , ZHANG Di , PENG Xinnuan , LIU Yanshan , SUN Jianrui , GONG Qiang . Potential mechanism of chaga mushroom in treatment of Alzheimer’s disease based on network pharmacology and molecular docking[J]. Food and Fermentation Industries, 2025 , 51(14) : 228 -235 . DOI: 10.13995/j.cnki.11-1802/ts.042100

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