In orderto construct a
genetic engineering phenylpyruvate reductase strain (E. coli/ppr), the expression conditions and bioconversion medium
were optimized to increase the production of PLA with whole-cell
transformation. A phenylpyruvate
reductase-encoding gene, lpppr, was obtained by PCR from the genomic DNA of Lactobacillus plantarum and heterologously expressed in E. coli BL21 (DE3). Taking phenylpyruvic
acid as the substrate, single factor and orthogonal experiments was used to
optimized the induced expression and whole-cell catalysis conditions. Results
showed that after inductionwith 0.5
mmol/L IPTG at 20 ℃
for 8 h, E. coli/ppr displayed the
highest LpPPR activity. When phenylpyruvic acid was added up to 25
mmol/L, the product enantiomeric excess percent was over 99.9% and the final
yield of PLA could reach 98.38% at 5 h under the obtained optimal
catalytic conditionsas
follows: temperature 40 ℃, initial pH 6.5 and 20 mmol/L glucose.
YUAN Feng-jiao et al
. Heterologous Expression of phenylpyruvate
reductase from Lactobacillus plantarum and Its Application in the Preparation of Phenyllactic Acid[J]. Food and Fermentation Industries, 2017
, 43(11)
: 16
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.015007