Food and Fermentation Industries >

2025 , Vol. 51 >Issue 13: 92 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040771

Protective effect of active extract of highland barley distiller’s grains on alcoholic liver injury in mice

  • FENG Xiaohuan ,
  • LUO Yihao ,
  • FENG Shengbao ,
  • SUN Wancheng ,
  • LI Shanwen
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  • 1(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
    2(Qinghai Huzhu Tianyoude Barley Wine Co.Ltd., Haidong 810700, China)

Received date: 2024-08-16

  Revised date: 2024-09-11

  Online published: 2025-08-04

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Abstract

To investigate the effects of different highland barley distiller’s grains (HBDG) active extracts on mice with alcoholic liver injury (ALI), HBDG polypeptides and HBDG polyphenols were extracted from HBDG powder, and ALI C57BL/6 J mice model was established through continuous astringent administration of 52° Tianyoude liquor for 14 days.The time of disappearance and recovery of intoxicated mice were observed and recorded;the morphopathological changes of liver were monitored by HE staining;serum and liver biochemical indices were measured by assay kit;and changes in liver metabolites were detected based on non-target metabolomics.Results showed that HBDG peptide and HBDG polyphenols intervention significantly reduced wake-up time (P<0.05) and improved inflammatory cell infiltration and lipid vacuolization caused by prolonged drinking.Compared with the control group, serum aspartate aminotransferase (AST) and triglyceride (TG) were significantly reduced (30.83%,44.35%) (P<0.05), while hepatic acetaldehyde dehydrogenase (ALDH) and superoxide dismutase (SOD) were remarkably increased (56.23%,49.96%) (P<0.05) in the HBDG peptide group.At this time, AST and TG of HBDG polyphenol experimental group were remarkably decreased (29.08%,33.87%) (P<0.05), and hepatic ALDH and SOD increased (12.80%,11.21%).In addition, ALDH and SOD levels were significantly improved (29.08%,33.87%) (P<0.05) in the experimental group compared with the HBDG polyphenol.Subsequently, the capacity of HBDG peptides to degrade alcohol and act as antioxidants surpassed that of HBDG polyphenols, showing a notable disparity (P<0.05).Analysis of liver metabolomics revealed that HBDG polypeptides could improve alcohol-induced liver damage by interfering with the arachidonic acid metabolic pathway to up-regulate the content of hepatic metabolites like prostaglandin F2α.Additionally, HBDG polyphenol might ameliorate alcohol-related liver by interfering with the linoleic acid metabolic to up-regulate 13-OxoODE and 8(R)-Hydroperoxylinoleic acid and impact thiamine metabolism to increase thiamine content, thereby alleviating alcohol-related liver harm.In summary, the use of HBDG peptides and HBDG polyphenols proved beneficial in enhancing ALI through the hastening of alcohol metabolism, bolstering antioxidant abilities, and controlling liver metabolic routes, with HBDG peptides outperforming HBDG polyphenols.The results can provide a theoretical basis for the study of new anti-alcohol and liver protection products.

Key words： highland barley distiller’s grains; polypeptide; polyphenol; alcoholic liver injury; hepatoprotective effect

Cite this article

FENG Xiaohuan , LUO Yihao , FENG Shengbao , SUN Wancheng , LI Shanwen . Protective effect of active extract of highland barley distiller’s grains on alcoholic liver injury in mice[J]. Food and Fermentation Industries, 2025 , 51(13) : 92 -100 . DOI: 10.13995/j.cnki.11-1802/ts.040771

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