Food and Fermentation Industries >

2025 , Vol. 51 >Issue 15: 41 - 50

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042337

Effect of Lactiplantibacillus plantarum GXFF202402 on the intervention of hemorrhoids in mice with irritable bowel syndrome

  • ZHAO Xin ,
  • TAN Xiaohong ,
  • LIU Xinhong ,
  • YI Ruokun ,
  • LONG Xingyao
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  • (Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China)

Received date: 2025-02-11

  Revised date: 2025-04-01

  Online published: 2025-08-22

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Abstract

This study investigated the therapeutic potential of Lactiplantibacillus plantarum GXFF202402 (LP-GXFF202402) in alleviating hemorrhoid-associated colorectal damage through gut microbiota modulation in a mouse model.In this study, croton oil was used to establish a mouse experimental model, and cytokine levels in serum and rectal tissues of mice were detected.Pathological observation of colon and rectal tissues of mice was carried out by hematoxylin-eosin (HE) staining.The relevant expression of microorganisms in tissue and intestinal contents was also detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot.The experimental results showed that LP-GXFF202402 could reduce the anorectal swelling rate of hemorrhoid mice.Improve serum IL-2 level of hemorrhoid mice and decrease serum levels of IL-4, IL-6, IL-1β, TNF-α and corticosterone (CORT).At the same time, it can also reduce the levels of IL-1β, IL-6, TNF-α, protease activated receptor 2 (PAR-2), mast cell tryptase (MCT) and increase the levels of IL-10, Occludin in rectal tissue of hemorrhoid mice.LP-GXFF202402 was also able to reduce the degree of colon and rectal lesions caused by hemorrhoids in mice.Further experimental results also showed that LP-GXFF202402 can up-regulate Occludin and down-regulate PAR-2 mRNA expression in colon tissue of hemorrhoid mice, and down-regulate IL-6 and TNF-α mRNA and protein expression in rectal tissue.The mRNA detection results of intestinal microorganisms showed that LP-GXFF202402 could increase the abundance of Firmicutes, Bifidobacteria and Lactobacillus and decrease the intensity of Bacteroidetes in the intestinal tract of hemorrhoid mice.It can be seen that LP-GXFF202402 can effectively intervene in the colorectal damage caused by hemorrhoids, regulate intestinal microbes, and thus relieve hemorrhoids, and the effect is close to the hemorrhoid drug Zhishuning tablet.

Key words： Lactiplantibacillus plantarum; irritable bowel syndrome; hemorrhoids; interleukin-6 (IL-6)

Cite this article

ZHAO Xin , TAN Xiaohong , LIU Xinhong , YI Ruokun , LONG Xingyao . Effect of Lactiplantibacillus plantarum GXFF202402 on the intervention of hemorrhoids in mice with irritable bowel syndrome[J]. Food and Fermentation Industries, 2025 , 51(15) : 41 -50 . DOI: 10.13995/j.cnki.11-1802/ts.042337

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