Food and Fermentation Industries >

2025 , Vol. 51 >Issue 15: 174 - 184

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040941

Expression and enzymatic properties of alginate lyase AlgL3199 from Vibrio sp.L2

  • WANG Haiying ,
  • CHEN Zhifang ,
  • ZHU Tiantian ,
  • SUN Jingjing ,
  • WANG Wei ,
  • HAO Jianhua
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  • 1(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China)
    2(College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China)
    3(College and Food Sciences and Technology, Shanghai Ocean University, Shanghai 200000, China)

Received date: 2024-09-03

  Revised date: 2024-11-05

  Online published: 2025-08-22

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Abstract

Alginate oligosaccharides with low molecular weight can be prepared by alginate lyase to overcome its disadvantage of low bioavailability and are widely used in medical, food, and cosmetics industries.AlgL3199, a gene-producing alginate lyase, was cloned from Vibrio sp.L2 genome, recombinant plasmid pET-24a (+)/AlgL3199 was constructed, and it was heterologously expressed.The enzymatic properties and degradation products of recombinant AlgL3199 were studied after purified with His TrapTM HP column affinity chromatography.The optimum temperature was 40 ℃, and the activity was retained more than 80% when it was kept at 10-35 ℃ for 1 h.The optimum pH value was 10, and above 70% activity could be retained after incubation for 12 h in a pH 8-10 buffer.Cu2+、Mn2+、Co2+ in 1 mmol/L could promote the activity of AlgL3199, while SDS and EDTA could inhibit its activity completely.AlgL3199 was most active with 1.125 mol/L NaCl.The thin layer chromatography showed the main products of PolyM, PolyG, and sodium alginate hydrolyzed by AlgL3199 were disaccharides, trisaccharides, and tetrasaccharide, suggesting AlgL3199 was a bifunctional endocytic enzyme with G preference.Study showed that AlgL3199 had been successfully expressed in E.coli and was stable at low temperature and weak alkalinity.It might be a new tool enzyme and an interesting candidate for the alginate industry and biotechnological applications.

Key words： alginate lyase; alginate oligosaccharide; enzymatic property; heterologous expression

Cite this article

WANG Haiying , CHEN Zhifang , ZHU Tiantian , SUN Jingjing , WANG Wei , HAO Jianhua . Expression and enzymatic properties of alginate lyase AlgL3199 from Vibrio sp.L2[J]. Food and Fermentation Industries, 2025 , 51(15) : 174 -184 . DOI: 10.13995/j.cnki.11-1802/ts.040941

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