Food and Fermentation Industries >

2025 , Vol. 51 >Issue 16: 10 - 19

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041306

Photodynamic inactivation and bactericidal mechanism of Escherichia coli O157∶H7 by using erythrosine B coupled with phage

  • REN Yu ,
  • SHI Hui
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  • (College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2024-10-15

  Revised date: 2024-11-09

  Online published: 2025-08-29

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Abstract

Photodynamic therapy (PDT) is an emerging non-thermal sterilization technology, but its application in the food industry is limited due to the lack of edible photosensitizer and excellent bactericidal effect.This study synthesized a conjugate (EBP) between the edible photosensitizer erythrosine B (EB) and the bacteriophage ZCFSTP4 (P4) to explore its bactericidal effect and mechanism.The conjugate EBP was synthesized from P4 and EB by EDC/NHS method.Escherichia coli O157∶H7 was used as the research object.Different light time and EBP concentration were optimized for sterilization conditions.Spectra were used to characterize the synthesis of EBP.The targeted recognition ability of EBP was detected by targeted experiments.The bactericidal mechanism of EBP was investigated by detecting the production capacity and species of reactive oxygen species (ROS), cell membrane permeability, cytoplasmic leakage, intracellular DNA, and protein content.Results showed that 15 μmol/L EBP had a synergistic antibactericidal effect under 60 min light condition.EBP was successfully synthesized and could specifically recognize Escherichia coli O157∶H7.EBP and EB had similar ROS generation capabilities, and ROS was mainly generated through type Ⅰ and type Ⅱ mechanisms, which exerted PDT effect to change cell membrane permeability and caused cytoplasmic leakage, resulting in a large reduction of intracellular DNA and protein.In conclusion, EBP could target bacteria and destroy the cell membrane structure by producing ROS, thus effectively killing bacteria.It provides a reference for the prevention and control of foodborne pathogens by PDT.

Key words： photodynamic sterilization; erythrosine B; bacteriophage; conjugate; Escherichia coli O157∶H7; mechanism of sterilization

Cite this article

REN Yu , SHI Hui . Photodynamic inactivation and bactericidal mechanism of Escherichia coli O157∶H7 by using erythrosine B coupled with phage[J]. Food and Fermentation Industries, 2025 , 51(16) : 10 -19 . DOI: 10.13995/j.cnki.11-1802/ts.041306

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