Food and Fermentation Industries >

2025 , Vol. 51 >Issue 16: 36 - 42

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041206

High yield expression of Ophiostoma piceae steryl esterase in Pichia pastoris

  • GUO Ziyan ,
  • MAO Wengang ,
  • LIN Hong ,
  • GAO Wenxin ,
  • WANG Yuefeng ,
  • SHU Zhengyu
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  • 1(National Joint Engineering Research Center of Industrial Microbiology and Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China)
    2(Weifang Key Laboratory of Respiratory Tract Pathogens and Drug Therapy, School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, China)

Received date: 2024-10-17

  Revised date: 2024-11-30

  Online published: 2025-08-29

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Abstract

The scarcity of microbial-derived steryl esterase resources restricts the popularization of green processing technology for phytosteryl modification.Ophistoma picae steryl esterase (OPE) is the first reported microbial-derived steryl esterase.The industrial production of OPE will effectively solve this technological bottleneck.To improve expression level of ope gene in Pichia pastoris, effect of ope gene copy numbers, secretion signal peptide types, and co-expression of protein disulfide isomerase on recombinant OPE yield was investigated in this study.Research results showed that all of the investigated factors played positive effect on OPE yield, and the highest yield of recombinant OPE was from the recombinant P.pastoris/pEESB-11, a coexpression system of protein disulfide isomerase gene with ope gene.The maximum expression level of ope gene from P.pastoris/pEESB-11 was 27.4 U/mL after the fermentation medium was optimized.This research work will greatly promote the application of OPE in the future.

Key words： Ophiostoma piceae steryl esterase; Pichia pastoris; gene multi-copy integration; signal peptide types; protein disulfide isomerase; high level heterogeneous expression

Cite this article

GUO Ziyan , MAO Wengang , LIN Hong , GAO Wenxin , WANG Yuefeng , SHU Zhengyu . High yield expression of Ophiostoma piceae steryl esterase in Pichia pastoris[J]. Food and Fermentation Industries, 2025 , 51(16) : 36 -42 . DOI: 10.13995/j.cnki.11-1802/ts.041206

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