Food and Fermentation Industries >

2025 , Vol. 51 >Issue 16: 43 - 50

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040863

The combined expression of rhamnose methylation related genes regulates synthesis of butenyl-spinosyn

  • SU Chang ,
  • GUO Chao ,
  • LI Xinying ,
  • CHEN Xia ,
  • PANG Jian ,
  • WANG Chao ,
  • ZHANG Genlin
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  • 1(School of Chemistry and Chemical Engineering, Shihezi University, State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi 832003, China)
    2(Academy of National Food and Strategic Reserves Administration, Beijing 100037, China)
    3(Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China)
    4(Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing 102206, China)

Received date: 2024-08-27

  Revised date: 2024-11-26

  Online published: 2025-08-29

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Abstract

The green biological insecticide butenyl-spinosyn has a broad spectrum and high-efficiency insecticidal activity and has great market prospects in crop production and grain storage, the breeding of strains with high production performance of butenyl-spinosyn is the key to realizing industrial production.In the biosynthesis of butenyl-spinosyn, S-adenosylmethionine (SAM) serves as a methyl donor and methylation rhamnose under the action of three different O-methyltransferases.The adequacy of O-methyltransferase and SAM supply is crucial for butenyl-spinosyn synthesis.Study investigated the impact of rhamnose methylation and related genes on butenyl-spinosyn synthesis by exogenously adding SAM, identifying and characterizing endogenous SAM synthase, metK, and O-methyltransferase gene cooperative expression.Results showed that the combined overexpression of metK and O-methyltransferase genes obtained three high-yielding engineering strains I-1, I-2, and H-1, and the yield was increased by 3.17, 2.21, and 1.43 times, respectively.In strain I-1, the overexpression of busI and metK1 regulated and promoted the strain’s growth metabolism and substrate consumption, thereby synthesizing butenyl-spinosyn more efficiently.The study provides a reference for the genetic engineering modification of Saccharopolyspora pogona to promote the synthesis of target products.

Key words： Saccharopolyspora pogona; butenyl-spinosyn; grain storage; pest control; O-methyltransferase; S-adenosylmethionine

Cite this article

SU Chang , GUO Chao , LI Xinying , CHEN Xia , PANG Jian , WANG Chao , ZHANG Genlin . The combined expression of rhamnose methylation related genes regulates synthesis of butenyl-spinosyn[J]. Food and Fermentation Industries, 2025 , 51(16) : 43 -50 . DOI: 10.13995/j.cnki.11-1802/ts.040863

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