Developing Colla Corii Asini and Ejiao cake as a health food has broad prospects.Based on the characterization of functional components, in vitro antioxidants, and cell experiments, the anti-skin aging effects of different polar solvent extracts of Colla Corii Asini and Ejiao cake were evaluated.Firstly, compared with other solvents (water, n-hexane, ethyl acetate), 70% ethanol solution was the most conducive to the dissolution of functional active ingredients such as sesamin, sesamol, and sesamin melanin in Ejiao cake.The peptides with a molecular weight of less than 500 Da accounted for 15.39% and 36.43% of the total peptides in the ethanol extracts of Colla Corii Asini and Ejiao cake, respectively.Compared with the ethanol extracts of Colla Corii Asini, the ethanol extracts of Ejiao cake had a higher proportion of antioxidant-active amino acids, such as glutamic acid, proline, and alanine.Secondly, antioxidant experiments showed that the ethanol extracts of Colla Corii Asini and Ejiao cake showed stronger ABTS cationic radical, DPPH free radical, hydroxyl radical, and superoxide anion scavenging effects than other extracts.Moreover, cell viability experiments showed that different extracts of Colla Corii Asini and Ejiao cake had no toxic effect on cell viability at the concentration range of 25-400 μg/mL.The cell senescence model was constructed by H2O2-induced oxidative stress.It was found that under the action of 200 μg/mL ethanol extract of Colla Corii Asini and Ejiao cake, the highest survival rates of damaged HaCaT cells were (84.16±3.21)% and (90.99±3.14)%, respectively.Thus, ethanol extract from Colla Corii Asini and Ejiao cake exhibited protective effects on the damaged cells.The scavenging rates of 200 μg/mL ethanol extract of Colla Corii Asini and Ejiao cake on reactive oxygen free radicals were (28.41±2.06)% and (41.83±2.81)% in damaged HaCaT cells, respectively.The results of this study provide a scientific basis for the application of Colla Corii Asini and Ejiao cake in the study of anti-cell senescence.
LI Cong
,
JI Mengmeng
,
MENG Lingfeng
,
MA Xueying
,
ZHANG Jianling
,
YUAN Yujiao
,
YU Qun
,
FAN Liuping
. Study on anti-skin aging effect of different polar solvent extracts of Colla Corii Asini and Ejiao cake[J]. Food and Fermentation Industries, 2025
, 51(17)
: 82
-92
.
DOI: 10.13995/j.cnki.11-1802/ts.041370
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