Food and Fermentation Industries >

2025 , Vol. 51 >Issue 18: 227 - 233

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041529

Study on physicochemical properties and hypoglycemic potential of polysaccharide from Tremella aurantialba

  • PAN Lichao ,
  • TU Yuanshu ,
  • LI Yuxin ,
  • WEI Zihua ,
  • LIU Li ,
  • ZHANG Aili ,
  • WU Ruobing ,
  • REN Haiwei ,
  • LIU Qiankui ,
  • WANG Yayu
Expand
  • 1(School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China)
    2(Gansu Industrial Technology Center for Food and Pharmaceutical Resources Development and Biomanufacturing, Lanzhou 730050, China)
    3(Gansu Ruikang Modern Biotechnology Limited Company, Lanzhou 730050, China)

Received date: 2024-11-07

  Revised date: 2025-01-23

  Online published: 2025-10-16

Fold

Abstract

Fruiting body of Tremella aurantialba cultivated in Lanzhou was used for experimental material.Tremella aurantialba pure polysaccharide (TAPP), an acidic heteropolysaccharide, was obtained from the material, with uniform molecular weight distribution (Mw=1.54×106 Da).Monosaccharide composition indicated that TAPP was composed of Man, GlcA, Glc, and Xyl with the ratio of 55.20∶9.76∶14.81∶20.23, and included a little Gal and GalA.The experiences of Fourier transform infrared spectroscopy, Congo red, scanning electron microscope, and thermogravimetric analysis showed that TAPP contained β-glycosidic bonds and helical structure, surface micromorphology presented lamellar and irregular aggregation, and it had good chemical stability below 170 ℃.The activity analysis showed that the inhibition of TAPP on α-amylase and α-glucosidase was positively correlated with concentration.The inhibitory effect on α-glucosidase was more significant, and the inhibition rate reached (93.32±1.09)% at TAPP concentration of 2.0 mg/mL.Its inhibition rate was similar to the effect of acarbose (P<0.05).It could significantly improve the insulin resistance of HepG2-IR model cells induced by palmitic acid when the concentration of TAPP reached 75 μg/mL (P<0.05).The results of this study provide theoretical supports and basis for further development and utilization of Tremella aurantialba resources in Lanzhou.

Key words： Tremella aurantialba; polysaccharide; separation and purification; structure analysis; hypoglycemic potential

Cite this article

PAN Lichao , TU Yuanshu , LI Yuxin , WEI Zihua , LIU Li , ZHANG Aili , WU Ruobing , REN Haiwei , LIU Qiankui , WANG Yayu . Study on physicochemical properties and hypoglycemic potential of polysaccharide from Tremella aurantialba[J]. Food and Fermentation Industries, 2025 , 51(18) : 227 -233 . DOI: 10.13995/j.cnki.11-1802/ts.041529

References

[1] 杨林雷, 李荣春, 曹瑶, 等. 金耳的学名及分类地位考证[J]. 食药用菌, 2020, 28(4):252-255; 276.
YANG L L, LI R C, CAO Y, et al. Research on the scientific name and taxonomic status of “Jin Er”[J]. Edible and Medicinal Mushrooms, 2020, 28(4):252-255; 276.
[2] 董昌金. 金耳菌丝体液体深层发酵和多糖提取研究[J]. 安徽农业科学, 2010, 38(1):342-344.
DONG C J. Study on the liquid-submerged fermentation and polysaccharide extraction of Tremella aurantialba mycelia[J]. Journal of Anhui Agricultural Sciences, 2010, 38(1):342-344.
[3] 康林芝, 林小乔, 金磊, 等. 金耳深层发酵培养基筛选和条件优化研究[J]. 食用菌, 2019, 41(2):13-15; 39.
KANG L Z, LIN X Q, JIN L, et al. Study on optimization of submerged fermentation medium and conditions for Tremella aurantialba Mycelium[J]. Edible Fungi, 2019, 41(2):13-15; 39.
[4] 刘子骐, 李众琪, 丁强, 等. 金耳酵母状孢子多糖双酶法提取工艺及其抗氧化性[J]. 食品研究与开发, 2023, 44(11):129-135;187.
LIU Z Q, LI Z Q, DING Q, et al. Dual-enzymatic extraction process and antioxidant activity of polysaccharide from yeast-like conidia of Naematelia aurantialba[J]. Food Research and Development, 2023, 44(11):129-135; 187.
[5] 李兴恺, 张耀根, 姚皓昱, 等. 毛韧革菌胞外多糖的结构表征、抗氧化活性研究及发酵条件优化[J]. 食品与发酵工业, 2022, 48(21):36-41.
LI X K, ZHANG Y G, YAO H Y, et al. Structure characterization, antioxidant activity and fermentation optimization of exopolysaccharide from Stereum hirsutum NX-22[J]. Food and Fermentation Industries, 2022, 48(21):36-41.
[6] 魏涛, 张静, 高兆兰. 金耳菌丝体多糖对高血糖小鼠血糖作用的研究[J]. 安徽农业科学, 2012, 40(12):7034-7035; 7052.
WEI T, ZHANG J, GAO Z L. Hypoglycemic activity of polysaccharides extracted from Tremella aurantialba Mycelium in experimental diabetic mice[J]. Journal of Anhui Agricultural Sciences, 2012, 40(12):7034-7035; 7052.
[7] 张雯, 赵旌旌, 王捷思, 等. 金耳菌丝体多糖对实验性2型糖尿病大鼠的降血糖作用研究[J]. 天然产物研究与开发, 2010, 22(1):49-53.
ZHANG W, ZHAO J J, WANG J S, et al. Hypoglycemic activity of polysaccharides extracted from Tremella aurantialba mycelia in experimental type 2 diabetic rats[J]. Natural Product Research and Development, 2010, 22(1):49-53.
[8] 黄年来, 吴经纶. 金耳菌种分离法初步研究[J]. 食用菌, 1983, 5(6):1-2.
HUANG N L, WU J L. Preliminary study on isolation method of Auricularia auricula strains[J]. Edible Fungi, 1983, 5(6):1-2.
[9] 田果廷, 赵丹丹, 赵永昌. 金耳有效菌种的制备技术研究[J]. 西南农业学报, 2010, 23(5):1620-1624.
TIAN G T, ZHAO D D, ZHAO Y C. Study on technology for spawn preparation for Tremella aurantialba[J]. Southwest China Journal of Agricultural Sciences, 2010, 23(5):1620-1624.
[10] 刘勇, 李春农. 金耳袋栽高产技术[J]. 农村科技开发, 1994(2):20.
LIU Y, LI C N. High-yield techniques of Auricularia auricula bag cultivation[J]. Rural science and technology development, 1994(2):20.
[11] PAN L C, ZHU Y M, ZHU Z Y, et al. Chemical structure and effects of antioxidation and against α-glucosidase of natural polysaccharide from Glycyrrhiza Inflata Batalin[J]. International Journal of Biological Macromolecules, 2020, 155:560-571.
[12] WANG Y P, GUO M R. Purification and structural characterization of polysaccharides isolated from Auricularia cornea var. Li[J]. Carbohydrate Polymers, 2020, 230:115680.
[13] JRIDI M, NASRI R, MARZOUGUI Z, et al. Characterization and assessment of antioxidant and antibacterial activities of sulfated polysaccharides extracted from cuttlefish skin and muscle[J]. International Journal of Biological Macromolecules, 2019, 123:1221-1228.
[14] 汤陈鹏, 吕峰, 王蓉琳. 孔石莼多糖锌结构表征与体外降血糖活性[J]. 食品科学, 2020, 41(7):52-58.
TANG C P, LÜ F, WANG R L. Structural characterization and hypoglycemic activity in vitro of Ulva pertusa polysaccharides-zinc complex[J]. Food Science, 2020, 41(7):52-58.
[15] WANG C, LI W W, CHEN Z Q, et al. Effects of simulated gastrointestinal digestion in vitro on the chemical properties, antioxidant activity, α-amylase and α-glucosidase inhibitory activity of polysaccharides from Inonotus obliquus[J]. Food Research International, 2018, 103:280-288.
[16] 陈建中, 葛水莲, 杨明建, 等. 太行菊黄酮对α-葡萄糖苷酶活性的抑制作用[J]. 食品与生物技术学报, 2023, 42(8):95-102.
CHEN J Z, GE S L, YANG M J, et al. Inhibition of the α-glucosidase activity by the flavonoids from Opisthopappus taihangensis (Ling) Shih[J]. Journal of Food Science and Biotechnology, 2023, 42(8):95-102.
[17] CHEN L, TENG H, CAO H. Chlorogenic acid and caffeic acid from Sonchus oleraceus Linn synergistically attenuate insulin resistance and modulate glucose uptake in HepG2 cells[J]. Food and Chemical Toxicology, 2019, 127:182-187.
[18] 李卫旗, 何国庆, 李志昂. 金耳水溶性多糖JP-2的分离及化学结构的研究[J]. 中国食品学报, 2003, 3(3):13-17.
LI W Q, HE G Q, LI Z A. Study on separation and chemical structure of water soluble polysaccharide JP-2 in Tremella aurantialba[J]. Journal of Chinese Institute of Food Science and Technology, 2003, 3(3):13-17.
[19] PENG G, WANG S S, ZHANG H S, et al. Tremella aurantialba polysaccharides alleviate ulcerative colitis in mice by improving intestinal barrier via modulating gut microbiota and inhibiting ferroptosis[J]. International Journal of Biological Macromolecules, 2024, 281:135835.
[20] XIE J H, WANG Z J, SHEN M Y, et al. Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus[J]. Food Hydrocolloids, 2016, 53:7-15.
[21] CHENG Y, XIE Y, GE J C, et al. Structural characterization and hepatoprotective activity of a galactoglucan from Poria cocos[J]. Carbohydrate Polymers, 2021, 263:117979.
[22] CHEN X, FANG D L, ZHAO R Q, et al. Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide[J]. International Journal of Biological Macromolecules, 2019, 140:505-514.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.