Food and Fermentation Industries >

2025 , Vol. 51 >Issue 20: 224 - 231

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042181

Preparation and properties of chitosan/lactic acid bacteria cell-free supernatant composite antibacterial film

  • YUAN Guohui ,
  • ZHAO Zicheng ,
  • DENG Yanmei ,
  • YANG Tongqin ,
  • GAO Baoyu ,
  • GUO Lei ,
  • YANG Jiaojiao ,
  • FAN Fangyu
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  • 1(College of Biological and Food Engineering, Southwest Forestry University, Kunming 650224, China)
    2(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China)
    3(Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, China)

Received date: 2025-01-17

  Revised date: 2025-03-19

  Online published: 2025-10-27

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Abstract

To improve the antibacterial properties of packaging films, the antibacterial and antioxidant properties of composite preservative solution made from varying ratios of chitosan (CS) and cell-free supernatant (CFS) of lactic acid bacteria were investigated.Composite packaging films were prepared by varying the volume ratios of CS to CFS (15∶0, 10∶1, 10∶2, 10∶3, 10∶4, 10∶5) were prepared using CS as the film-forming matrix.The effects of different CFS ratios on the morphology, structure, mechanical properties, and barrier properties of the films were evaluated.The antibacterial and antioxidant effects of the composite preservative solution were positively proportional to the additional amount of CFS, reaching their maximum values at a CS to CFS ratio of 10∶5.The inhibition zone diameters against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was 14.68, 15.83, and 13.40 mm, respectively.DPPH free radical and ABTS cationic radical scavenging rates were 94.46% and 92.24%.With the increase in CFS content, the composite film exhibited improved light barrier properties compared to the pure CS film, along with increased thickness, water vapor permeability, water solubility, and water content, while CO2 permeability decreased.The mechanical properties and thermal stability of the composite film were optimal at a CS to CFS ratio of 10∶3.The tensile strength and elongation at break increased by 36.77% and 70.94% compared with the pure CS film (P＜0.05), and the melting temperature reached 125.28 ℃.Characterization analysis showed that the composite film exhibited excellent compatibility, dense structure, and enhanced stability.

Key words： lactic acid bacteria; chitosan; cell-free supernatant; composite film; antibacterial

Cite this article

YUAN Guohui , ZHAO Zicheng , DENG Yanmei , YANG Tongqin , GAO Baoyu , GUO Lei , YANG Jiaojiao , FAN Fangyu . Preparation and properties of chitosan/lactic acid bacteria cell-free supernatant composite antibacterial film[J]. Food and Fermentation Industries, 2025 , 51(20) : 224 -231 . DOI: 10.13995/j.cnki.11-1802/ts.042181

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