Dynamic succession of yeast flora during Cabernet Franc spontaneous fermentation in Penglai Coastal Sub-region

  • GAO Yue ,
  • YU Di ,
  • ZHANG Xue ,
  • LIU Layu ,
  • CHEN Songbo ,
  • AN Peng ,
  • LIU Yanlin ,
  • JIANG Jiao
Expand
  • 1(College of Enology, Northwest A&F University, Yangling 712100, China)
    2(Longting Vineyard, Penglai 265600, China)
    3(Chateau Reifeng Auzias, Penglai 265600, China)
    4(Heyang Experimental and Demonstration Stations for Grape, Northwest A&F University, Weinan 715300, China)
    5(Ningxia Helan Mountain’s East Foothill Wine Experiment and Demonstration Station of Northwest A&F University, Yongning 750100, China)

Received date: 2025-01-10

  Revised date: 2025-02-19

  Online published: 2025-11-03

Abstract

Indigenous wild yeast communities exhibit distinct regional variations that contribute more effectively to the flavour uniqueness of the wine compared to commercial yeasts.This study aimed to investigate the succession patterns of indigenous yeast diversity in the spontaneous fermentation system of Cabernet Franc grapes from the Penglai Coast sub-region.Yeast isolation and identification were conducted during the natural fermentation using grapes from two vineyards in this sub-region over two consecutive years.Saccharomyces cerevisiae isolates were then analysed for their genotypes by Interdelta Fingerprinting.The results show that 387 yeast strains were isolated and purified during spontaneous fermentation of Cabernet Franc wines across two vintages.These strains belonged to 3 genera and 20 species.Among them, 248 strains were identified as S.cerevisiae.These S.cerevisiae strains were further catagorized into 34 genotypes.Significant variations in yeast species diversity and genetic diversity of S.cerevisiae were observed between vintages and vineyards.This study provides valuable insights into the development and utilization of indigenous yeast strains for Cabernet Franc wine production in Penglai.

Cite this article

GAO Yue , YU Di , ZHANG Xue , LIU Layu , CHEN Songbo , AN Peng , LIU Yanlin , JIANG Jiao . Dynamic succession of yeast flora during Cabernet Franc spontaneous fermentation in Penglai Coastal Sub-region[J]. Food and Fermentation Industries, 2025 , 51(19) : 56 -62 . DOI: 10.13995/j.cnki.11-1802/ts.042102

References

[1] 张春芝, 江志国.微生物对葡萄酒香气的影响综述[J].中国酿造, 2013, 32(9):28-31.
ZHANG C Z, JIANG Z G.Review of microbial effects on wine aroma[J].China Brewing, 2013, 32(9):28-31.
[2] 徐超, 胡文效, 陈明光, 等.葡萄酒发酵香气物质调控研究进展[J].中外葡萄与葡萄酒, 2024(1):81-93.
XU C, HU W X, CHEN M G, et al.Advances on regulation of aroma substances in wine fermentation[J].Sino-Overseas Grapevine & Wine, 2024(1):81-93.
[3] GURAKAN G C, AKTUNA I, SEYEDMONIR E.Diversity of wild yeasts during spontaneous fermentation of wines from local grape varieties in Turkey[J].American Journal of Enology and Viticulture, 2022, 73(4):308-320.
[4] BAGHERI B, PHILIPP C, HORACEK M, et al.Microbial diversity in grape musts from Austrian and South African grape varieties and regions[J].BIO Web of Conferences, 2019, 12:02028.
[5] DRUMONDE-NEVES J, FRANCO-DUARTE R, VIEIRA E, et al.Differentiation of Saccharomyces cerevisiae populations from vineyards of the Azores Archipelago:Geography vs Ecology[J].Food Microbiology, 2018, 74:151-162.
[6] CAPECE A, ROMANIELLO R, SIESTO G, et al.Diversity of Saccharomyces cerevisiae yeasts associated to spontaneously fermenting grapes from an Italian “heroic vine-growing area”[J].Food Microbiology, 2012, 31(2):159-166.
[7] SUN Y, QIN Y, PEI Y F, et al.Evaluation of Chinese Saccharomyces cerevisiae wine strains from different geographical origins[J].American Journal of Enology and Viticulture, 2017, 68(1):73-80.
[8] LI R L, LIN M Y, GUO S J, et al.A fundamental landscape of fungal biogeographical patterns across the main Chinese wine-producing regions and the dominating shaping factors[J].Food Research International, 2021, 150:110736.
[9] 魏如腾. 葡萄-葡萄酒自然体系中微生物多样性及其对葡萄酒风味物质的作用[D].杨凌:西北农林科技大学, 2023.
WEI R T.Microbial diversity in grape-wine natural systems and its role in wine flavour compounds[D].Yangling:Northwest A&F University, 2023.
[10] 汤晓宏. 蓬莱葡萄酒产区酿酒酵母的选育—中粮君顶酒庄酿酒酵母的筛选[D].济南:齐鲁工业大学, 2014.
TANG X H.The breeding of Saccharomyces cerevisiae in Penglai wine region—The screening of Saccharomyces cerevisiae of Chateau junding[D].Jinan:Qilu University of Technology, 2014.
[11] WANG C P, CHEN X Q, REN Y H, et al.Author Correction:Geographical location influence ‘Cabernet Franc’ fruit quality in Shandong province[J].Scientific Reports, 2024, 14:7942.
[12] LIANG L H, MA Y W, JIANG Z Z, et al.Dynamic analysis of microbial communities and flavor properties in Merlot wines produced from inoculation and spontaneous fermentation[J].Food Research International, 2023, 164:112379.
[13] COSTANTINI A, VAUDANO E, PULCINI L, et al.Yeast biodiversity in vineyard during grape ripening:Comparison between culture dependent and NGS analysis[J].Processes, 2022, 10(5):901.
[14] 刘佳禾, 王蕊, 周叶丽, 等.宁夏贺兰山东麓产区维欧尼葡萄酒自然发酵过程中酵母菌群多样性研究[J].中国酿造, 2024, 43(7):73-78.
LIU J H, WANG R, ZHOU Y L, et al.Diversity of yeast community during spontaneous fermentation of Viognier wine from the eastern foothills of Helan Mountain in Ningxia[J].China Brewing, 2024, 43(7):73-78.
[15] 周小玲, 沈微, 饶志明, 等.一种快速提取真菌染色DNA的方法[J].微生物学通报, 2004, 31(4):89-92.
ZHOU X L, SHEN W, RAO Z M, et al.A rapid method for preparation of fungal chromosome DNA[J].Microbiology, 2004, 31(4):89-92.
[16] KUMAR S, STECHER G, LI M, et al.MEGA X:Molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution, 2018, 35(6):1547-1549.
[17] 杨慧敏, 任晓娜, 秦义.四川甘孜州高山葡萄酒产区酵母菌多样性分析[J].微生物学通报, 2022, 49(7):2673-2685.
YANG H M, REN X N, QIN Y.Diversity analysis of yeasts in the Ganzi alpine wine region, Sichuan[J].Microbiology China, 2022, 49(7):2673-2685.
[18] FENG L, WANG J M, YE D Q, et al.Yeast population dynamics during spontaneous fermentation of icewine and selection of indigenous Saccharomyces cerevisiae strains for the winemaking in Qilian, China[J].Journal of the Science of Food and Agriculture, 2020, 100(15):5385-5394.
[19] HUANG R, JIANG J, SU Y, et al.Microbial community succession and volatile compounds changes during spontaneous fermentation of Cabernet Sauvignon (Vitis vinifera L.) under rain-shelter cultivation[J].Food Chemistry:X, 2023, 17:100618.
[20] ŠURANSKÁ H, VRÁNOVÁ D, OMELKOVÁ J.Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains[J].Brazilian Journal of Microbiology, 2016, 47(1):181-190.
[21] CHEN Y, QI J L, YANG H Y, et al.Fungal dynamic during apricot wine spontaneous fermentation and aromatic characteristics of Pichia kudriavzevii for potential as starter[J].Food Chemistry:X, 2023, 19:100862.
[22] VAN LEEUWEN C, SEGUIN G.The concept of terroir in viticulture[J].Journal of Wine Research, 2006, 17(1):1-10.
[23] MARTÍNEZ C, COSGAYA P, VÁSQUEZ C, et al.High degree of correlation between molecular polymorphism and geographic origin of wine yeast strains[J].Journal of Applied Microbiology, 2007, 103(6):2185-2195.
Outlines

/

〈 〉