The κ-carrageenan(KC)-eugenol(EUG) films were successfully fabricated by incorporating EUG into KC matrix via casting.The effects of different EUG contents (5%, 10%, 15%) on the morphology, structure, mechanical properties, barrier properties, and surface wettability of the films were systematically investigated.It was found that EUG and KC were bonded to each other through hydrogen bonding, and the flexibility, barrier properties and hydrophobicity of the films were enhanced with the increase of EUG content.In addition, the KC-EUG films also possessed good antioxidant and antimicrobial activity, which were proportional to the EUG content.Given the volatility of EUG, the encapsulation rate of EUG in the KC-EUG15 film was measured.The results indicated that the actual encapsulation efficiency of EUG in the KC-EUG15 film reached (68.2±1.1)%, demonstrating a favorable encapsulation effect.The practical application evaluation demonstrated that KC-EUG15 film possessed good antimicrobial properties through both direct contact and volatilization modes, and extended the shelf life of milk by nearly 100% at 25 ℃.In conclusion, the addition of EUG significantly optimizes the physical and functional properties of KC-EUG films, showing great potential in the field of antimicrobial packaging.
WU Yimin
,
LIU Chenlong
,
GE Zhiyuan
,
WANG Zhongzhi
,
LI Linlin
. Preparation and application of κ-carrageenan-eugenol films[J]. Food and Fermentation Industries, 2025
, 51(19)
: 146
-153
.
DOI: 10.13995/j.cnki.11-1802/ts.043309
[1] LI Z X, RAWDKUEN S, FEI T, et al.Recent advances in gellan gum-based films and its application in sustainable food packaging:A review[J].Critical Reviews in Food Science and Nutrition, 2025:1-22.
[2] ROMAINOR A N, CHIN S F, LIHAN S.Antimicrobial starch-based film for food packaging application[J].Starch-Stärke, 2022, 74(3-4):2100207.
[3] GOPALAKRISHNAN K, CHANDEL M, GUPTA V, et al.Valorisation of fruit peel bioactive into green synthesized silver nanoparticles to modify cellulose wrapper for shelf-life extension of packaged bread[J].Food Research International, 2023, 164:112321.
[4] PRIYADARSHI R, RHIM J W.Chitosan-based biodegradable functional films for food packaging applications[J].Innovative Food Science & Emerging Technologies, 2020, 62:102346.
[5] HAN Y Y, YU M, WANG L J.Preparation and characterization of antioxidant soy protein isolate films incorporating licorice residue extract[J].Food Hydrocolloids, 2018, 75:13-21.
[6] WU X, LIU Z, HE S, et al.Development of an edible food packaging gelatin/zein based nanofiber film for the shelf-life extension of strawberries[J].Food Chemistry, 2023, 426:136652.
[7] SHANKAR S, BANG Y J, RHIM J W.Antibacterial LDPE/GSE/Mel/ZnONP composite film-coated wrapping paper for convenience food packaging application[J].Food Packaging and Shelf Life, 2019, 22:100421.
[8] SANTINON C, BEPPU M M, VIEIRA M G A.Optimization of kappa-carrageenan cationization using experimental design for model-drug release and investigation of biological properties[J].Carbohydrate Polymers, 2023, 308:120645.
[9] LOMOLINO G, VINCENZI S, ZANNONI S, et al.Emulsifying activity of potato proteins in the presence of κ-carrageenan at different pH conditions[J].Food Chemistry:X, 2022, 13:100232.
[10] XIE H J, XIANG C Y, LI Y, et al.Fabrication of ovalbumin/κ-carrageenan complex nanoparticles as a novel carrier for curcumin delivery[J].Food Hydrocolloids, 2019, 89:111-121.
[11] ZHENG H X, MAO L K, CUI M N, et al.Development of food-grade bigels based on κ-carrageenan hydrogel and monoglyceride oleogels as carriers for β-carotene:Roles of oleogel fraction[J].Food Hydrocolloids, 2020, 105:105855.
[12] SAEDI S, SHOKRI M, RHIM J W.Preparation of carrageenan-based nanocomposite films incorporated with functionalized halloysite using AgNP and sodium dodecyl sulfate[J].Food Hydrocolloids, 2020, 106:105934.
[13] KHAN A, RIAHI Z, KIM J T, et al.Gelatin/carrageenan-based smart packaging film integrated with Cu-metal organic framework for freshness monitoring and shelf-life extension of shrimp[J].Food Hydrocolloids, 2023, 145:109180.
[14] LIU J R, WANG H L, GUO M, et al.Extract from Lycium ruthenicum Murr.Incorporating κ-carrageenan colorimetric film with a wide pH-sensing range for food freshness monitoring[J].Food Hydrocolloids, 2019, 94:1-10.
[15] OVERLY K R.Microwave-assisted isolation of eugenol from cloves[J].Journal of Chemical Education, 2019, 96(11):2665-2667.
[16] FU X, GAO Y, YAN W Y, et al.Preparation of eugenol nanoemulsions for antibacterial activities[J].RSC Advances, 2022, 12(6):3180-3190.
[17] LIU J R, WANG H L, WANG P F, et al.Films based on κ-carrageenan incorporated with curcumin for freshness monitoring[J].Food Hydrocolloids, 2018, 83:134-142.
[18] LI L L, XIA L, XIAO F, et al.Colorimetric active carboxymethyl chitosan/oxidized sodium alginate-Oxalis triangularis ssp.papilionacea anthocyanins film@gelatin/zein-linalool membrane for milk freshness monitoring and preservation[J].Food Chemistry, 2023, 405(Pt B):134994.
[19] ZHAO R N, CHEN J, YU S F, et al.Active chitosan/gum arabic-based emulsion films reinforced with thyme oil encapsulating blood orange anthocyanins:Improving multi-functionality[J].Food Hydrocolloids, 2023, 134:108094.
[20] WANG H, YUAN D, MENG Q R, et al.Pickering nanoemulsion loaded with eugenol contributed to the improvement of konjac glucomannan film performance[J].International Journal of Biological Macromolecules, 2024, 267:131495.
[21] ROY S, SHANKAR S, RHIM J W.Melanin-mediated synthesis of silver nanoparticle and its use for the preparation of carrageenan-based antibacterial films[J].Food Hydrocolloids, 2019, 88:237-246.
[22] CHENG J F, WANG H L, KANG S L, et al.An active packaging film based on yam starch with eugenol and its application for pork preservation[J].Food Hydrocolloids, 2019, 96:546-554.
[23] MOAZAMI GOODARZI M, MORADI M, TAJIK H, et al.Development of an easy-to-use colorimetric pH label with starch and carrot anthocyanins for milk shelf life assessment[J].International Journal of Biological Macromolecules, 2020, 153:240-247.
[24] GAO R C, HU H L, SHI T, et al.Incorporation of gelatin and Fe2+ increases the pH-sensitivity of zein-anthocyanin complex films used for milk spoilage detection[J].Current Research in Food Science, 2022, 5:677-686.
[25] ZHENG Y W, LI X M, HUANG Y, et al.Two colorimetric films based on chitin whiskers and sodium alginate/gelatin incorporated with anthocyanins for monitoring food freshness[J].Food Hydrocolloids, 2022, 127:107517.
