To expand the application of novel bacterial cellulose (BC) in composite bacteriostatic materials, this study employed microbial fermentation to produce bacterial cellulose.Subsequently, bacterial cellulose/microbial lysozyme/sodium phytate composite membranes were prepared using an in-situ generation-solution immersion method.These membranes were then evaluated for their physical properties, bacteriostatic activity, and cytocompatibility.Results indicated that the incorporation of sodium phytate reduced the porosity of the composite membrane.Notably, the water swelling equilibrium of the composite membrane reached its peak (about 6 313%) with the addition of 0.01% sodium phytate, demonstrating significant hydrophilicity. Furthermore, the tensile strength of the composite membrane exhibited a 2.36-fold increase, while the elongation at break was enhanced by 85.37%, respectively, compared to BC alone. The composite membrane also exhibited remarkable bacteriostatic properties, inhibiting the growth of Candida albicans, Salmonella, and Bacillus subtilis by more than 70%.Additionally, the composite membrane demonstrated excellent biocompatibility and blood compatibility.Overall, the bacterial cellulose/microbial lysozyme/sodium phytate composite membranes developed in this study exhibit superior biological antimicrobial properties, suggesting a broad potential for development and application in the biomedical field.
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