To investigate the effect of glycerol monolaurate (GML) microemulsion on delaying spoilage and deterioration caused by special spoilage organisms (SSOs) in refrigerated large yellow croaker, the inhibitory properties of GML microemulsion against single/mixed SSOs (Shewanella baltica,Pseudomonas brenneri) were firstly investigated in vitro.Large yellow croaker blocks inoculated with SSOs were treated with GML microemulsion to examine the in vivo bacteriostatic effect by examining the quality indicators during storage.The results found that the bacteriostatic activity of GML microemulsion on the SSOs was obvious.The inhibition rate of extracellular protease and the diameter of the inhibition circle increased, which exacerbated the leakage of intracellular proteins and nucleic acids, causing changes in cell membrane permeability.At the same time, there was also some damage to the cell wall structure, with folds and constrictions on the cell surface and intercellular adhesions.The inhibitory effect of a high concentration of GML microemulsion on S.baltica was better than that of P.brenneri, in which the inhibitory effect of 1.5 mg/mL GML microemulsion on the mixed bacterial suspension would be more obvious than that in the presence of alone.Results of in vivo inoculation experiment showed that when large yellow croaker blocks were in mixed inoculation mode of SSOs, 1.5 mg/mL GML microemulsion significantly delayed the increase of total viable count, deterioration of sensory characteristics, alteration of ammonia content, and amino acid decarboxylase activity, and degradation of myofibrillar protein.The rate of increase of TCA-soluble peptide content was also slowed down.In conclusion, 1.5 mg/mL GML microemulsion could be used to retard spoilage deterioration of refrigerated large yellow croakers caused by SSOs.
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