Food and Fermentation Industries >

2025 , Vol. 51 >Issue 19: 174 - 182

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041584

Preparation and performance study of nanoemulsion loaded with thyme essential oil, soybean oil, and thymol

  • WEI Jinhuan ,
  • WANG Zikun ,
  • LU Rui ,
  • YANG Jinjie ,
  • ZHANG Songyan ,
  • HUA Chun ,
  • XIN Ying ,
  • CHEN Fusheng
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  • (College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China)

Received date: 2024-11-13

  Revised date: 2025-02-15

  Online published: 2025-11-03

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Abstract

To augment the stability and antimicrobial efficacy of thyme essential oil nanoemulsion, this study investigated the fabrication process of nanoemulsion (MONE) incorporating a thyme essential oil/soybean oil /thymol mixture as the oil phase.The influence of various parameters, including the pH of the aqueous phase, the mass ratio of whey protein isolate (WPI) to low methoxyl pectin (LMP), the concentration of the WPI-LMP complex, the volume fraction of the oil phase, and the thymol content, on the mean particle size and encapsulation rate of MONE were meticulously examined.Additionally, the stability and antimicrobial properties of the nanoemulsion were assessed.Findings indicated that under optimal conditions, specifically, an aqueous phase pH of 5.0, a WPI-to-LMP mass ratio of 1∶2, a WPI-LMP complex concentration of 25 g/L, an oil phase volume fraction of 25 mL, and a thymol addition corresponding to 5% of the oil phase volume, the MONE exhibited a mean particle diameter of (513.0±2.6) nm with an encapsulation rate of (93.00±0.36)%.Stability trials demonstrated that MONE maintained its stability at 4 ℃ for 30 days, within a pH range from 3 to 7, and under sodium ion concentrations of 0-400 mmol/L.Furthermore, compared to the essential oil nanoemulsion without thymol, MONE showed an approximately 8% increase in the inhibition zones against Escherichia coli and Staphylococcus aureus, and its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for both tested strains were 15.62 μL/mL and 31.25 μL/mL, respectively, indicating that MONE exhibits good antibacterial activity.The outcomes of this research lay a foundational groundwork for the development of nanoemulsions with a mixed oil phase and their potential applications in the realm of food preservation.

Key words： mixed oil phase nanoemulsion; soybean oil; thymol; stability; antimicrobial activity

Cite this article

WEI Jinhuan , WANG Zikun , LU Rui , YANG Jinjie , ZHANG Songyan , HUA Chun , XIN Ying , CHEN Fusheng . Preparation and performance study of nanoemulsion loaded with thyme essential oil, soybean oil, and thymol[J]. Food and Fermentation Industries, 2025 , 51(19) : 174 -182 . DOI: 10.13995/j.cnki.11-1802/ts.041584

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