Food and Fermentation Industries >

2025 , Vol. 51 >Issue 21: 43 - 52

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042935

Effects of Lactobacillus brevis CQPC12 on endurance and running function in mice

  • LI Ke ,
  • SONG Canlin ,
  • TIAN Xiaoxue ,
  • ZHAO Xin
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  • 1(College of Physical Education and Health Management, Chongqing University of Education, Chongqing 400065, China)
    2(Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing University of Education, Chongqing 400067, China)

Received date: 2025-04-06

  Revised date: 2025-06-03

  Online published: 2025-11-21

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Abstract

This study explored the role of Lactobacillus brevis CQPC12 (LB-CQPC12) in improving the running performance of mice with oxidative damage and regulating the related molecular mechanisms.The in vitro resistance test results of LB-CQPC12 showed that this strain had excellent acid resistance and bile salt tolerance characteristics.In vivo experiments were conducted to explore the physiological regulatory effect of LB-CQPC12 on running exercise in mice with oxidative damage induced by D-galactose.The experimental observation indicators included endurance test, serum biochemical analysis, histopathological examination, and detection of key gene mRNA expression levels in gastrocnemius muscle and liver tissue.The results indicated that oral administration of LB-CQPC12 at a dose of 1.5×109 CFU/kg BW significantly enhanced running performance in mice with oxidative damage (P<0.05). This improvement was evidenced by a notable prolongation of running time, significant reductions in lactic acid (LA), blood urea nitrogen (BUN), and creatine kinase (CK) levels, along with markedly increased hepatic glycogen (HG) and muscle glycogen (MG) concentrations (P<0.05). Histopathological analysis confirmed that LB-CQPC12 could effectively alleviate oxidative stress injury in the liver.At the molecular level, LB-CQPC12 significantly upregulated peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) compared with the model control group.glutathione peroxidase 1 (GPx1), which can activate adenylate activated protein kinase, AMPK) and manganese superoxide dismutase (Mn-SOD) (P<0.05), and upregulated the expression of AMPKα1, PGC1- α/β protein expressions.In addition, LB-CQPC12 significantly reduced the number of harmful bacteria Bacteroidetes and increased the number of the other three beneficial bacteria in the intestinal contents.This study shows that LB-CQPC12 can not only significantly improve the exercise endurance under oxidative injury, but also enhance the running ability of mice.Its effect is significantly better than that of the traditional antioxidant ascorbic acid (150 mg/kg BW gavage).This result provides an important theoretical basis for the development of sports nutritional supplements based on lactic acid bacteria.

Key words： Lactobacillus brevis; endurance; running function; oxidative stress; mice

Cite this article

LI Ke , SONG Canlin , TIAN Xiaoxue , ZHAO Xin . Effects of Lactobacillus brevis CQPC12 on endurance and running function in mice[J]. Food and Fermentation Industries, 2025 , 51(21) : 43 -52 . DOI: 10.13995/j.cnki.11-1802/ts.042935

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